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Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines
State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China
Institute of Crop Science, the National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 18 January 2013; in revised form: 5 April 2013 / Accepted: 9 April 2013 / Published: 16 April 2013
Abstract: Small brown planthopper (SBPH) and rice stripe virus (RSV) disease transmitted by SBPH cause serious damage to rice (Oryza sativa L.) in China. In the present study, we screened 312 rice accessions for resistance to SBPH. The indica variety, N22, is highly resistant to SBPH. One hundred and eighty two recombinant inbred lines (RILs) derived from a cross of N22 and the highly susceptible variety, USSR5, were used for quantitative trait locus (QTL) analysis of resistances to SBPH and RSV. In a modified seedbox screening test, three QTLs for SBPH resistance, qSBPH2, qSBPH3 and qSBPH7.1, were mapped on chromosomes 2, 3 and 7, a total explaining 35.1% of the phenotypic variance. qSBPH7.2 and qSBPH11.2, conferring antibiosis against SBPH, were detected on chromosomes 7 and 11 and accounted for 20.7% of the total phenotypic variance. In addition, qSBPH5 and qSBPH7.3, expressing antixenosis to SBPH, were detected on chromosomes 5 and 7, explaining 23.9% of the phenotypic variance. qSBPH7.1, qSBPH7.2 and qSBPH7.3, located in the same region between RM234 and RM429 on chromosome 7, using three different phenotyping methods indicate that the locus or region plays a major role in conferring resistance to SBPH in N22. Moreover, three QTLs, qSTV4, qSTV11.1 and qSTV11.2, for RSV resistance were detected on chromosomes 4 and 11. qSTV11.1 and qSTV11.2 are located in the same region between RM287 and RM209 on chromosome 11. Molecular markers spanning these QTLs should be useful in the development of varieties with resistance to SBPH and RSV.
Keywords: RIL population; quantitative trait locus; Oryza sativa L.
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Wang, Q.; Liu, Y.; Hu, J.; Zhang, Y.; Xie, K.; Wang, B.; Tuyen, L.Q.; Song, Z.; Wu, H.; Liu, Y.; Jiang, L.; Liu, S.; Cheng, X.; Wang, C.; Zhai, H.; Wan, J. Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines. Int. J. Mol. Sci. 2013, 14, 8406-8421.
Wang Q, Liu Y, Hu J, Zhang Y, Xie K, Wang B, Tuyen LQ, Song Z, Wu H, Liu Y, Jiang L, Liu S, Cheng X, Wang C, Zhai H, Wan J. Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines. International Journal of Molecular Sciences. 2013; 14(4):8406-8421.
Wang, Qi; Liu, Yuqiang; Hu, Jinlong; Zhang, Yingxin; Xie, Kun; Wang, Baoxiang; Tuyen, Le Q.; Song, Zhaoqiang; Wu, Han; Liu, Yanling; Jiang, Ling; Liu, Shijia; Cheng, Xianian; Wang, Chunming; Zhai, Huqu; Wan, Jianmin. 2013. "Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines." Int. J. Mol. Sci. 14, no. 4: 8406-8421.