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Int. J. Mol. Sci. 2013, 14(4), 7853-7865; doi:10.3390/ijms14047853
Article

Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons

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Received: 1 March 2013; in revised form: 29 March 2013 / Accepted: 2 April 2013 / Published: 10 April 2013
(This article belongs to the Special Issue Redox Signaling in Biology and Patho-Biology)
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Abstract: Oxidative stress has been implicated in the pathogenesis of Friedreich’s Ataxia (FRDA), a neurodegenerative disease caused by the decreased expression of frataxin, a mitochondrial protein responsible of iron homeostasis. Under conditions of oxidative stress, the activation of the transcription factor NF-E2-related factor (Nrf2) triggers the antioxidant cellular response by inducing antioxidant response element (ARE) driven genes. Increasing evidence supports a role for the Nrf2-ARE pathway in neurodegenerative diseases. In this study, we analyzed the expression and the distribution of Nrf2 in silenced neurons for frataxin gene. Decreased Nrf2 mRNA content and a defective activation after treatment with pro-oxidants have been evidenced in frataxin-silenced neurons by RT-PCR and confocal microscopy. The loss of Nrf2 in FRDA may greatly enhance the cellular susceptibility to oxidative stress and make FRDA neurons more vulnerable to injury. Our findings may help to focus on this promising target, especially in its emerging role in the neuroprotective response.
Keywords: FRDA; frataxin; Nrf2; GSSG; oxidative stress; NSC34 neurons FRDA; frataxin; Nrf2; GSSG; oxidative stress; NSC34 neurons
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

D'Oria, V.; Petrini, S.; Travaglini, L.; Priori, C.; Piermarini, E.; Petrillo, S.; Carletti, B.; Bertini, E.; Piemonte, F. Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons. Int. J. Mol. Sci. 2013, 14, 7853-7865.

AMA Style

D'Oria V, Petrini S, Travaglini L, Priori C, Piermarini E, Petrillo S, Carletti B, Bertini E, Piemonte F. Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons. International Journal of Molecular Sciences. 2013; 14(4):7853-7865.

Chicago/Turabian Style

D'Oria, Valentina; Petrini, Stefania; Travaglini, Lorena; Priori, Chiara; Piermarini, Emanuela; Petrillo, Sara; Carletti, Barbara; Bertini, Enrico; Piemonte, Fiorella. 2013. "Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons." Int. J. Mol. Sci. 14, no. 4: 7853-7865.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert