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Int. J. Mol. Sci. 2013, 14(4), 7506-7514; doi:10.3390/ijms14047506
Article

Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides

1,2
,
1,2,*  and 3,*
1 State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China 2 Institute for Technology Research and Innovation of Sun Yat-sen University, Zhuhai 519000, China 3 CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
* Authors to whom correspondence should be addressed.
Received: 14 January 2013 / Revised: 3 March 2013 / Accepted: 26 March 2013 / Published: 8 April 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

CVN (cyanovirin-N) is an anti-HIV protein. CVNH (cyanovirin-N homology) represents its homology. In a previous study, we first reported the full-length sequences of the CVNH gene cloned from Ceratopteris thalictroides. Based on the finding, the coding sequence of CtCVNH was optimized in the study, and then a pET prokaryotic expression vector was constructed. The purification and identification of CtCVNH protein were investigated, as well. SDS-PAGE analysis indicated that a 31 kDa protein was overexpressed and mainly accumulated in the soluble fraction. Only a single protein was obtained after the Ni- nitrilotriacetic acid (NTA) affinity chromatography. The purified protein was identified to be the recombinant CtCVNH by both Western blot and peptide mass fingerprinting analysis.
Keywords: Ceratopteris thalictroides; CtCVNH; anti-HIV protein; prokaryotic expression Ceratopteris thalictroides; CtCVNH; anti-HIV protein; prokaryotic expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sun, J.; Su, Y.; Wang, T. Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides. Int. J. Mol. Sci. 2013, 14, 7506-7514.

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