Int. J. Mol. Sci. 2013, 14(3), 6157-6169; doi:10.3390/ijms14036157
Article

LC-MS/MS Determination of Isoprostanes in Plasma Samples Collected from Mice Exposed to Doxorubicin or Tert-Butyl Hydroperoxide

1 Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland 2 Tri-City Central Animal Laboratory Research and Service Centre of the Medical University of Gdansk, Medical University of Gdansk, 80-210 Gdańsk, Poland 3 Department of Food Chemistry, Technology and Biotechnology, Chemical Faculty, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
* Author to whom correspondence should be addressed.
Received: 10 January 2013; in revised form: 5 February 2013 / Accepted: 6 March 2013 / Published: 18 March 2013
(This article belongs to the Special Issue Oxidative Stress and Ageing)
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Abstract: Isoprostanes are stable products of arachidonic acid peroxidation and are regarded as the most reliable markers of oxidative stress in vivo. Here we describe the LC-MS/MS procedure enabling simultaneous determination of four regioisomers (8-iso prostaglandin F, 8-iso-15(R)-prostaglandin F, 11β-prostaglandin F, 15(R)-prostaglandin F) in plasma samples collected from mice. The four plasma isoprostanes are determined by LC–ESI-MS/MS with deuterated 8-iso-PGF-d4 as an internal standard (I.S.). For plasma samples spiked with the isoprostanes at a level of 200 pg/mL each, the method imprecision has been below 7.1% and mean inaccuracy equaled 8.7%. The applicability of the proposed approach has been verified by the assessment of changes in isoprostane levels in plasma samples derived from mice exposed to tert-butyl hydroperoxide (TBHP), a model inducer of oxidative stress, or to antitumor drug doxorubicin (DOX) known for potent stimulation of redox cycling. Compared to the control group of mice, both oxidative stress inducers tested increased the levels of three out of four isoprostanes in exposed animals; 11β-prostaglandin F being the exception. The greatest rise was observed in the case of 15(R)-prostaglandin F, by about 50% and 70% in plasma samples derived from mice exposed to DOX and TBHP, respectively.
Keywords: isoprostanes; doxorubicin; plasma biomarkers of oxidative stress; LC-MS/MS

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MDPI and ACS Style

Janicka, M.; Kot-Wasik, A.; Paradziej-Łukowicz, J.; Sularz-Peszyńska, G.; Bartoszek, A.; Namieśnik, J. LC-MS/MS Determination of Isoprostanes in Plasma Samples Collected from Mice Exposed to Doxorubicin or Tert-Butyl Hydroperoxide. Int. J. Mol. Sci. 2013, 14, 6157-6169.

AMA Style

Janicka M, Kot-Wasik A, Paradziej-Łukowicz J, Sularz-Peszyńska G, Bartoszek A, Namieśnik J. LC-MS/MS Determination of Isoprostanes in Plasma Samples Collected from Mice Exposed to Doxorubicin or Tert-Butyl Hydroperoxide. International Journal of Molecular Sciences. 2013; 14(3):6157-6169.

Chicago/Turabian Style

Janicka, Monika; Kot-Wasik, Agata; Paradziej-Łukowicz, Jolanta; Sularz-Peszyńska, Grażyna; Bartoszek, Agnieszka; Namieśnik, Jacek. 2013. "LC-MS/MS Determination of Isoprostanes in Plasma Samples Collected from Mice Exposed to Doxorubicin or Tert-Butyl Hydroperoxide." Int. J. Mol. Sci. 14, no. 3: 6157-6169.

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