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Structure, Function and Networks of Transcription Factors Involved in Abiotic Stress Responses
Int. J. Mol. Sci. 2013, 14(3), 5899-5919; doi:10.3390/ijms14035899

A Rice Immunophilin Gene, OsFKBP16-3, Confers Tolerance to Environmental Stress in Arabidopsis and Rice

2 and 1,*
1 Green Bio Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-506, Korea 2 Department of Pharmacology, Medical Science, Seonam University, Namwon 590-170, Korea 3 Division of Bio-Crops Development, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 30 January 2013 / Revised: 4 March 2013 / Accepted: 5 March 2013 / Published: 13 March 2013
(This article belongs to the Special Issue Abiotic and Biotic Stress Tolerance Mechanisms in Plants)
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The putative thylakoid lumen immunophilin, FKBP16-3, has not yet been characterized, although this protein is known to be regulated by thioredoxin and possesses a well-conserved CxxxC motif in photosynthetic organisms. Here, we characterized rice OsFKBP16-3 and examined the role of this gene in the regulation of abiotic stress in plants. FKBP16-3s are well conserved in eukaryotic photosynthetic organisms, including the presence of a unique disulfide-forming CxxxC motif in their N-terminal regions. OsFKBP16-3 was mainly expressed in rice leaf tissues and was upregulated by various abiotic stresses, including salt, drought, high light, hydrogen peroxide, heat and methyl viologen. The chloroplast localization of OsFKBP16-3-GFP was confirmed through the transient expression of OsFKBP16-3 in Nicotiana benthamiana leaves. Transgenic Arabidopsis and transgenic rice plants that constitutively expressed OsFKBP16-3 exhibited increased tolerance to salinity, drought and oxidative stresses, but showed no change in growth or phenotype, compared with vector control plants, when grown under non-stressed conditions. This is the first report to demonstrate the potential role of FKBP16-3 in the environmental stress response, which may be regulated by a redox relay process in the thylakoid lumen, suggesting that artificial regulation of FKBP16-3 expression is a candidate for stress-tolerant crop breeding.
Keywords: FK506 binding protein; FKBP16-3; thylakoid lumen; environmental stress tolerance FK506 binding protein; FKBP16-3; thylakoid lumen; environmental stress tolerance
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Park, H.J.; Lee, S.S.; You, Y.N.; Yoon, D.H.; Kim, B.-G.; Ahn, J.C.; Cho, H.S. A Rice Immunophilin Gene, OsFKBP16-3, Confers Tolerance to Environmental Stress in Arabidopsis and Rice. Int. J. Mol. Sci. 2013, 14, 5899-5919.

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