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Int. J. Mol. Sci. 2013, 14(2), 4361-4371; doi:10.3390/ijms14024361
Article

Prevention of Tendon Adhesions by ERK2 Small Interfering RNAs

,
,
,
 and *
These authors contributed equally to this work. Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, School of Medicine, 600 Yishan Road, Shanghai 200233, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 21 November 2012 / Revised: 6 January 2013 / Accepted: 7 January 2013 / Published: 21 February 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013)
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Abstract

Tendon adhesions are one of the most concerning complications after surgical repair of flexor tendon injury. Extracellular signal-regulated kinase (ERK) 2 plays crucial roles in fibroblast proliferation and collagen expression which contributes to the formation of tendon adhesions after flexor tendon surgery. Using a chicken model, we have examined the effects of a small interfering RNA (siRNA) targeting ERK2 delivered by a lentiviral system on tendon adhesion formation with an adhesion scoring system, histological assessment, and biomechanical evaluation. It was found that ERK2 siRNA effectively suppressed the increase of fibroblasts and the formation of tendon adhesions (p < 0.05 compared with the control group). Moreover, no statistically significant reduction in breaking force was detected between the ERK2 siRNA group and the control group. These results show that the lentiviral-mediated siRNA system is effective in preventing tendon adhesion formation but not to tendon healing, and may be used for tendon repair after confirmation and improvement by future detailed studies.
Keywords: tendon repair; adhesion; siRNA; extracellular signal-regulated kinase (ERK) 2; fibroblast proliferation; lentivirus tendon repair; adhesion; siRNA; extracellular signal-regulated kinase (ERK) 2; fibroblast proliferation; lentivirus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ruan, H.; Liu, S.; Li, F.; Li, X.; Fan, C. Prevention of Tendon Adhesions by ERK2 Small Interfering RNAs. Int. J. Mol. Sci. 2013, 14, 4361-4371.

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