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Int. J. Mol. Sci. 2013, 14(2), 4283-4297; doi:10.3390/ijms14024283
Article

Effect of Nanoencapsulated Vitamin B1 Derivative on Inhibition of Both Mycelial Growth and Spore Germination of Fusarium oxysporum f. sp. raphani

1
, 2
, 1
 and 3,*
Received: 20 December 2012; in revised form: 23 January 2013 / Accepted: 29 January 2013 / Published: 21 February 2013
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Abstract: Nanoencapsulation of thiamine dilauryl sulfate (TDS), a vitamin B1 derivative, was proved to effectively inhibit the spore germination of Fusarium oxysporum f. sp. raphani (F. oxysporum), as well as mycelial growth. The average diameter of nanoparticles was measured as 136 nm by being encapsulated with an edible encapsulant, lecithin, whose encapsulation efficiency was about 55% in containing 200 ppm of TDS concentration: the 100 ppm TDS nanoparticle solution showed a mycelial growth inhibition rate of 59%. These results were about similar or even better than the cases of treating 100 ppm of dazomet, a positive antifungal control (64%). Moreover, kinetic analysis of inhibiting spore germination were estimated as 6.6% reduction of spore germination rates after 24 h treatment, which were 3.3% similar to the case of treating 100 ppm of a positive control (dazomet) for the same treatment time. It was also found that TDS itself could work as an antifungal agent by inhibiting both mycelial growth and spore germination, even though its efficacy was lower than those of nanoparticles. Nanoparticles especially played a more efficient role in limiting the spore germination, due to their easy penetration into hard cell membranes and long resident time on the surface of the spore shell walls. In this work, it was first demonstrated that the nanoparticle of TDS not a harmful chemical can control the growth of F. oxysporum by using a lower dosage than commercial herbicides, as well as the inhibiting mechanism of the TDS. However, field trials of the TDS nanoparticles encapsulated with lecithin should be further studied to be effectively used for field applications.
Keywords: thiamine dilauryl sulfate; nanoencapsulation; lecithin; spore germination; mycelial growth; Fusarium oxysporum f. sp. raphani thiamine dilauryl sulfate; nanoencapsulation; lecithin; spore germination; mycelial growth; Fusarium oxysporum f. sp. raphani
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cho, J.S.; Seo, Y.C.; Yim, T.B.; Lee, H.Y. Effect of Nanoencapsulated Vitamin B1 Derivative on Inhibition of Both Mycelial Growth and Spore Germination of Fusarium oxysporum f. sp. raphani. Int. J. Mol. Sci. 2013, 14, 4283-4297.

AMA Style

Cho JS, Seo YC, Yim TB, Lee HY. Effect of Nanoencapsulated Vitamin B1 Derivative on Inhibition of Both Mycelial Growth and Spore Germination of Fusarium oxysporum f. sp. raphani. International Journal of Molecular Sciences. 2013; 14(2):4283-4297.

Chicago/Turabian Style

Cho, Jeong S.; Seo, Yong C.; Yim, Tae B.; Lee, Hyeon Y. 2013. "Effect of Nanoencapsulated Vitamin B1 Derivative on Inhibition of Both Mycelial Growth and Spore Germination of Fusarium oxysporum f. sp. raphani." Int. J. Mol. Sci. 14, no. 2: 4283-4297.



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