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Int. J. Mol. Sci. 2013, 14(2), 2875-2902; doi:10.3390/ijms14022875
Article

Optimization of β-Glucosidase, β-Xylosidase and Xylanase Production by Colletotrichum graminicola under Solid-State Fermentation and Application in Raw Sugarcane Trash Saccharification

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Received: 5 September 2012; in revised form: 12 December 2012 / Accepted: 9 January 2013 / Published: 30 January 2013
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Abstract: Efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. The production of β-glucosidase, β-xylosidase and xylanase by Colletotrichum graminicola was optimized using Response Surface Methodology (RSM). Maximal production occurred in wheat bran. Sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and xylanase production, respectively. Maximal levels after optimization reached 159.3 ± 12.7 U g−1, 128.1 ± 6.4 U g−1 and 378.1 ± 23.3 U g−1, respectively, but the enzymes were produced simultaneously at good levels under culture conditions optimized for each one of them. Optima of pH and temperature were 5.0 and 65 °C for the three enzymes, which maintained full activity for 72 h at 50 °C and for 120 min at 60 °C (β-glucosidase) or 65 °C (β-xylosidase and xylanase). Mixed with Trichoderma reesei cellulases, C. graminicola crude extract hydrolyzed raw sugarcane trash with glucose yield of 33.1% after 48 h, demonstrating good potential to compose efficient cocktails for lignocellulosic materials hydrolysis.
Keywords: β-glucosidase; β-xylosidase; xylanase; Colletotrichum graminicola; sugarcane trash hydrolysis β-glucosidase; β-xylosidase; xylanase; Colletotrichum graminicola; sugarcane trash hydrolysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zimbardi, A.L.R.L.; Sehn, C.; Meleiro, L.P.; Souza, F.H.M.; Masui, D.C.; Nozawa, M.S.F.; Guimarães, L.H.S.; Jorge, J.A.; Furriel, R.P.M. Optimization of β-Glucosidase, β-Xylosidase and Xylanase Production by Colletotrichum graminicola under Solid-State Fermentation and Application in Raw Sugarcane Trash Saccharification. Int. J. Mol. Sci. 2013, 14, 2875-2902.

AMA Style

Zimbardi ALRL, Sehn C, Meleiro LP, Souza FHM, Masui DC, Nozawa MSF, Guimarães LHS, Jorge JA, Furriel RPM. Optimization of β-Glucosidase, β-Xylosidase and Xylanase Production by Colletotrichum graminicola under Solid-State Fermentation and Application in Raw Sugarcane Trash Saccharification. International Journal of Molecular Sciences. 2013; 14(2):2875-2902.

Chicago/Turabian Style

Zimbardi, Ana L.R.L.; Sehn, Cesar; Meleiro, Luana P.; Souza, Flavio H.M.; Masui, Douglas C.; Nozawa, Monica S.F.; Guimarães, Luis H.S.; Jorge, João A.; Furriel, Rosa P.M. 2013. "Optimization of β-Glucosidase, β-Xylosidase and Xylanase Production by Colletotrichum graminicola under Solid-State Fermentation and Application in Raw Sugarcane Trash Saccharification." Int. J. Mol. Sci. 14, no. 2: 2875-2902.


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