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Int. J. Mol. Sci. 2013, 14(2), 2449-2483; doi:10.3390/ijms14022449
Article

Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors

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1 The Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov Str., Ekaterinburg 620014, Russia 2 The Ural State Medical Academy, 17 Klyuchevskaya Str., Ekaterinburg 620109, Russia 3 The Institute of Natural Sciences, the Ural Federal University, Ekaterinburg 620000, Russia 4 The City Clinical Diagnostics Centre, 38 Dekabristov Str., Ekaterinburg 620142, Russia
* Author to whom correspondence should be addressed.
Received: 9 November 2012 / Revised: 8 January 2013 / Accepted: 9 January 2013 / Published: 25 January 2013
(This article belongs to the Special Issue Bioactive Nanoparticles 2012)
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Abstract

Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat’s pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a “bioprotective complex” (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS.
Keywords: nanosilver; nanogold; bronchoalveolar lavage; subchronic toxicity; genotoxicity; bioprotectors nanosilver; nanogold; bronchoalveolar lavage; subchronic toxicity; genotoxicity; bioprotectors
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Katsnelson, B.A.; Privalova, L.I.; Gurvich, V.B.; Makeyev, O.H.; Shur, V.Y.; Beikin, Y.B.; Sutunkova, M.P.; Kireyeva, E.P.; Minigalieva, I.A.; Loginova, N.V.; Vasilyeva, M.S.; Korotkov, A.V.; Shuman, E.A.; Vlasova, L.A.; Shishkina, E.V.; Tyurnina, A.E.; Kozin, R.V.; Valamina, I.E.; Pichugova, S.V.; Tulakina, L.G. Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors. Int. J. Mol. Sci. 2013, 14, 2449-2483.

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