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Expression Pattern of Class B Gene PAP3 in Flower Development of Pepper
Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, China
* Author to whom correspondence should be addressed.
Received: 30 October 2013; in revised form: 24 November 2013 / Accepted: 3 December 2013 / Published: 18 December 2013
Abstract: Class B gene APETALA3 (AP3) plays a key role in the development of petals and stamens. Here, we investigated the expression pattern of PAP3 gene (genbank accession number: HM104635) in the buds of cytoplasmic male sterility line 121A and its near-isogenic restorer line 121C at four developmental stages and analyzed the possible association between Class B genes and cytoplasmic male sterility of pepper. Semi-quantitative PCR and quantitative real-time RT-PCR (qRT-PCR) as well as RNA in situ hybridization showed increased expression of PAP3 at late phase of anther development and its higher expression in restorer line compared with sterility line indicating PAP3’s role at late developmental stage of anther and suppressed expression in sterility line. RNA in situ hybridization showed Class B gene features: high abundance in stamen and petal; lower expression in pistil; no expression in sepal. Results of transient expression in onion epidermal cells also showed PAP3 localized in the nucleus, which is consistent with the expression pattern of transcription factors of MADS-box gene family.
Keywords: pepper; PAP3; expression analyses; subcellular localization
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MDPI and ACS Style
Li, X.; Liu, C.; Da, F.; Ma, N.; Shen, H. Expression Pattern of Class B Gene PAP3 in Flower Development of Pepper. Int. J. Mol. Sci. 2013, 14, 24643-24655.
Li X, Liu C, Da F, Ma N, Shen H. Expression Pattern of Class B Gene PAP3 in Flower Development of Pepper. International Journal of Molecular Sciences. 2013; 14(12):24643-24655.
Li, Xin; Liu, Chen; Da, Fengjiao; Ma, Ning; Shen, Huolin. 2013. "Expression Pattern of Class B Gene PAP3 in Flower Development of Pepper." Int. J. Mol. Sci. 14, no. 12: 24643-24655.