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Open AccessCommunication
Int. J. Mol. Sci. 2013, 14(12), 24046-24054; doi:10.3390/ijms141224046

Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.

1,2,†
,
2,3,†
,
1,2,†
,
2,* and 1,2
1
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China
2
Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
3
Qingdao University of Science and Technology, Qingdao 266042, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 October 2013 / Revised: 23 November 2013 / Accepted: 29 November 2013 / Published: 10 December 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

A Gram-negative strain (TJ) capable of growing aerobically on mixed phthalate esters (PAEs) as the sole carbon and energy source was isolated from the Haihe estuary, Tianjin, China. It was identified as belonging to the Sphingobium genus on the basis of morphological and physiological characteristics and 16S rRNA and gyrb gene sequencing. The batch tests for biodegradation of di-n-butyl phthalate (DBP) by the Sphingobium sp. TJ showed that the optimum conditions were 30 °C, pH 7.0, and the absence of NaCl. Stain TJ could tolerate up to 4% NaCl in minimal salt medium supplemented with DBP, although the DBP degradation rates slowed as NaCl concentration increased. In addition, substrate tests showed that strain TJ could utilize shorter side-chained PAEs, such as dimethyl phthalate and diethyl phthalate, but could not metabolize long-chained PAEs, such as di-n-octyl phthalate, diisooctyl phthalate, and di-(2-ethyl-hexyl) phthalate. To our knowledge, this is the first report on the biodegradation characteristics of DBP by a member of the Sphingobium genus. View Full-Text
Keywords: di-n-butyl phthalate; biodegradation; halotolerant; Sphingobium sp.; 16S rRNA gene; gyrb gene di-n-butyl phthalate; biodegradation; halotolerant; Sphingobium sp.; 16S rRNA gene; gyrb gene
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jin, D.; Kong, X.; Cui, B.; Bai, Z.; Zhang, H. Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.. Int. J. Mol. Sci. 2013, 14, 24046-24054.

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