Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2013, 14(12), 24046-24054; doi:10.3390/ijms141224046
Communication

Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.

1,2,†
, 2,3,†
, 1,2,†
, 2,*  and 1,2
Received: 9 October 2013; in revised form: 23 November 2013 / Accepted: 29 November 2013 / Published: 10 December 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [796 KB, uploaded 19 June 2014]
Abstract: A Gram-negative strain (TJ) capable of growing aerobically on mixed phthalate esters (PAEs) as the sole carbon and energy source was isolated from the Haihe estuary, Tianjin, China. It was identified as belonging to the Sphingobium genus on the basis of morphological and physiological characteristics and 16S rRNA and gyrb gene sequencing. The batch tests for biodegradation of di-n-butyl phthalate (DBP) by the Sphingobium sp. TJ showed that the optimum conditions were 30 °C, pH 7.0, and the absence of NaCl. Stain TJ could tolerate up to 4% NaCl in minimal salt medium supplemented with DBP, although the DBP degradation rates slowed as NaCl concentration increased. In addition, substrate tests showed that strain TJ could utilize shorter side-chained PAEs, such as dimethyl phthalate and diethyl phthalate, but could not metabolize long-chained PAEs, such as di-n-octyl phthalate, diisooctyl phthalate, and di-(2-ethyl-hexyl) phthalate. To our knowledge, this is the first report on the biodegradation characteristics of DBP by a member of the Sphingobium genus.
Keywords: di-n-butyl phthalate; biodegradation; halotolerant; Sphingobium sp.; 16S rRNA gene; gyrb gene di-n-butyl phthalate; biodegradation; halotolerant; Sphingobium sp.; 16S rRNA gene; gyrb gene
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Jin, D.; Kong, X.; Cui, B.; Bai, Z.; Zhang, H. Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.. Int. J. Mol. Sci. 2013, 14, 24046-24054.

AMA Style

Jin D, Kong X, Cui B, Bai Z, Zhang H. Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.. International Journal of Molecular Sciences. 2013; 14(12):24046-24054.

Chicago/Turabian Style

Jin, Decai; Kong, Xiao; Cui, Bingjian; Bai, Zhihui; Zhang, Hongxun. 2013. "Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.." Int. J. Mol. Sci. 14, no. 12: 24046-24054.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert