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Int. J. Mol. Sci. 2013, 14(12), 23711-23735; doi:10.3390/ijms141223711
Article

Mutation in the pssA Gene Involved in Exopolysaccharide Synthesis Leads to Several Physiological and Symbiotic Defects in Rhizobium leguminosarum bv. trifolii

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Received: 7 October 2013; in revised form: 14 November 2013 / Accepted: 14 November 2013 / Published: 5 December 2013
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Abstract: The symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum bv. trifolii 24.2 secretes large amounts of acidic exopolysaccharide (EPS), which plays a crucial role in establishment of effective symbiosis with clover. The biosynthesis of this heteropolymer is conducted by a multi-enzymatic complex located in the bacterial inner membrane. PssA protein, responsible for the addition of glucose-1-phosphate to a polyprenyl phosphate carrier, is involved in the first step of EPS synthesis. In this work, we characterize R. leguminosarum bv. trifolii strain Rt270 containing a mini-Tn5 transposon insertion located in the 3'-end of the pssA gene. It has been established that a mutation in this gene causes a pleiotropic effect in rhizobial cells. This is confirmed by the phenotype of the mutant strain Rt270, which exhibits several physiological and symbiotic defects such as a deficiency in EPS synthesis, decreased motility and utilization of some nutrients, decreased sensitivity to several antibiotics, an altered extracellular protein profile, and failed host plant infection. The data of this study indicate that the protein product of the pssA gene is not only involved in EPS synthesis, but also required for proper functioning of Rhizobium leguminosarum bv. trifolii cells.
Keywords: pssA mutant; exopolysaccharide synthesis; metabolic profile; motility; Rhizobium leguminosarum bv. trifolii; symbiosis; clover pssA mutant; exopolysaccharide synthesis; metabolic profile; motility; Rhizobium leguminosarum bv. trifolii; symbiosis; clover
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Janczarek, M.; Rachwał, K. Mutation in the pssA Gene Involved in Exopolysaccharide Synthesis Leads to Several Physiological and Symbiotic Defects in Rhizobium leguminosarum bv. trifolii. Int. J. Mol. Sci. 2013, 14, 23711-23735.

AMA Style

Janczarek M, Rachwał K. Mutation in the pssA Gene Involved in Exopolysaccharide Synthesis Leads to Several Physiological and Symbiotic Defects in Rhizobium leguminosarum bv. trifolii. International Journal of Molecular Sciences. 2013; 14(12):23711-23735.

Chicago/Turabian Style

Janczarek, Monika; Rachwał, Kamila. 2013. "Mutation in the pssA Gene Involved in Exopolysaccharide Synthesis Leads to Several Physiological and Symbiotic Defects in Rhizobium leguminosarum bv. trifolii." Int. J. Mol. Sci. 14, no. 12: 23711-23735.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert