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Int. J. Mol. Sci. 2013, 14(10), 20326-20339; doi:10.3390/ijms141020326
Article

MicroRNA Expression Profiling of the Porcine Developing Hypothalamus and Pituitary Tissue

1,†
, 2,†
, 3,†
, 1
, 1
, 1
 and 1,*
Received: 19 August 2013; in revised form: 17 September 2013 / Accepted: 23 September 2013 / Published: 14 October 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: MicroRNAs (miRNAs), a class of small non-coding RNA molecules, play important roles in gene expressions at transcriptional and post-transcriptional stages in mammalian brain. So far, a growing number of porcine miRNAs and their function have been identified, but little is known regarding the porcine developing hypothalamus and pituitary. In the present study, Solexa sequencing analysis showed 14,129,397 yielded reads, 6,680,678 of which were related to 674 unique miRNAs. After a microarray assay, we detected 175 unique miRNAs in the hypothalamus, including 136 previously known miRNAs and 39 novel candidates, while a total of 140 miRNAs, including 104 known and 36 new candidate miRNAs, were discovered in pituitary. More importantly, 37 and 30 differentially expressed miRNAs from several developmental stages of hypothalamus and pituitary were revealed, respectively. The 37 differentially expressed miRNAs in hypothalamus represented 6 different expression patterns, while the 30 differentially expressed miRNAs in pituitary represented 7 different expression patterns. To clarify potential target genes and specific functions of these differentially expressed miRNAs in hypothalamus and pituitary, TargetScan and Gorilla prediction tools were then applied. The current functional analysis showed that the differentially expressed miRNAs in hypothalamus and pituitary shared many biological processes, with the main differences being found in tissue-specific processes including: CDP-diacylglycerol biosynthetic/metabolic process; phosphatidic acid biosynthetic/metabolic process; energy reserve metabolic process for hypothalamus; adult behavior; sterol transport/homeostasis; and cholesterol/reverse cholesterol transport for pituitary. Overall, this study identified miRNA profiles and differentially expressed miRNAs among various developmental stages in hypothalamus and pituitary and indicated miRNA profiles change with age and brain location, enhancing our knowledge about spatial and temporal expressions of miRNAs in the porcine developing brain.
Keywords: miRNA; Solexa sequencing; microarray; hypothalamus; pituitary; pig miRNA; Solexa sequencing; microarray; hypothalamus; pituitary; pig
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, L.; Cai, Z.; Wei, S.; Zhou, H.; Zhou, H.; Jiang, X.; Xu, N. MicroRNA Expression Profiling of the Porcine Developing Hypothalamus and Pituitary Tissue. Int. J. Mol. Sci. 2013, 14, 20326-20339.

AMA Style

Zhang L, Cai Z, Wei S, Zhou H, Zhou H, Jiang X, Xu N. MicroRNA Expression Profiling of the Porcine Developing Hypothalamus and Pituitary Tissue. International Journal of Molecular Sciences. 2013; 14(10):20326-20339.

Chicago/Turabian Style

Zhang, Lifan; Cai, Zhaowei; Wei, Shengjuan; Zhou, Huiyun; Zhou, Hongmei; Jiang, Xiaoling; Xu, Ningying. 2013. "MicroRNA Expression Profiling of the Porcine Developing Hypothalamus and Pituitary Tissue." Int. J. Mol. Sci. 14, no. 10: 20326-20339.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert