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Int. J. Mol. Sci. 2013, 14(10), 20299-20325; doi:10.3390/ijms141020299
Article

Physiological and Proteomic Responses of Diploid and Tetraploid Black Locust (Robinia pseudoacacia L.) Subjected to Salt Stress

1,†
, 1,†
, 2
 and 1,*
Received: 17 June 2013; in revised form: 31 August 2013 / Accepted: 9 September 2013 / Published: 14 October 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
Abstract: Tetraploid black locust (Robinia pseudoacacia L.) is adaptable to salt stress. Here, we compared morphological, physiological, ultrastructural, and proteomic traits of leaves in tetraploid black locust and its diploid relatives under salt stress. The results showed that diploid (2×) plants suffered from greater negative effects than those of tetraploid (4×) plants. After salt treatment, plant growth was inhibited, photosynthesis was reduced, reactive oxygen species, malondialdehyde content, and relative electrolyte leakage increased, and defense-related enzyme activities decreased in 2× compared to those in 4×. In addition, salt stress resulted in distorted chloroplasts, swollen thylakoid membranes, accumulation of plastoglobules, and increased starch grains in 2× compared to those in 4×. However, 4× developed diverse responses under salt stress. A comparative proteomic analysis revealed that 41 and 37 proteins were differentially expressed in 2× and 4×, respectively. These proteins were mainly involved in photosynthesis, stress and defense, energy, metabolism, transcription/translation, and transportation. Distinct patterns of protein changes between 2× and 4× were analyzed. Collectively, our results suggest that the plants showed significantly different responses to salt stress based on ploidy level of the plant. The 4× possessed a better salt protection mechanism than that of 2×, suggesting salt tolerance in the polyploid plant.
Keywords: salt stress; Robinia pseudoacacia L.; diploid; tetraploid; physiology; proteomics salt stress; Robinia pseudoacacia L.; diploid; tetraploid; physiology; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Z.; Wang, M.; Liu, L.; Meng, F. Physiological and Proteomic Responses of Diploid and Tetraploid Black Locust (Robinia pseudoacacia L.) Subjected to Salt Stress. Int. J. Mol. Sci. 2013, 14, 20299-20325.

AMA Style

Wang Z, Wang M, Liu L, Meng F. Physiological and Proteomic Responses of Diploid and Tetraploid Black Locust (Robinia pseudoacacia L.) Subjected to Salt Stress. International Journal of Molecular Sciences. 2013; 14(10):20299-20325.

Chicago/Turabian Style

Wang, Zhiming; Wang, Mingyue; Liu, Likun; Meng, Fanjuan. 2013. "Physiological and Proteomic Responses of Diploid and Tetraploid Black Locust (Robinia pseudoacacia L.) Subjected to Salt Stress." Int. J. Mol. Sci. 14, no. 10: 20299-20325.


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