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Int. J. Mol. Sci. 2013, 14(1), 1655-1666; doi:10.3390/ijms14011655
Article

Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents

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Received: 17 December 2012; in revised form: 7 January 2013 / Accepted: 10 January 2013 / Published: 15 January 2013
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment.
Keywords: kadsuralignan F; melanogenesis; tyrosinase degradation kadsuralignan F; melanogenesis; tyrosinase degradation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Goh, M.-J.; Lee, H.-K.; Cheng, L.; Kong, D.-Y.; Yeon, J.-H.; He, Q.-Q.; Cho, J.-C.; Na, Y.J. Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents. Int. J. Mol. Sci. 2013, 14, 1655-1666.

AMA Style

Goh M-J, Lee H-K, Cheng L, Kong D-Y, Yeon J-H, He Q-Q, Cho J-C, Na YJ. Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents. International Journal of Molecular Sciences. 2013; 14(1):1655-1666.

Chicago/Turabian Style

Goh, Myeong-Jin; Lee, Hae-Kwang; Cheng, Liang; Kong, De-Yun; Yeon, Jae-Ho; He, Quan-Quan; Cho, Jun-Cheol; Na, Yong J. 2013. "Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents." Int. J. Mol. Sci. 14, no. 1: 1655-1666.



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