Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2012, 13(8), 9864-9876; doi:10.3390/ijms13089864
Article

Acute Effect of Ghrelin on Ischemia/Reperfusion Injury in the Rat Spinal Cord

1,2,†
, 2,†
, 2
, 2
, 2
 and 2,*
Received: 18 May 2012; in revised form: 17 July 2012 / Accepted: 19 July 2012 / Published: 8 August 2012
View Full-Text   |   Download PDF [925 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Ghrelin, a 28-amino acid peptide, is mainly secreted by the stomach. Ghrelin has been shown to have neuroprotective effects. However, whether ghrelin protects the spinal cord from ischemia/reperfusion (I/R) injury is unknown. To investigate this, 60 rats were randomly divided into three different groups: the sham group (n = 20), the vehicle group (n = 20), and the Ghrelin group (100 µg/kg, n = 20). Rats were sacrificed 12, 24, 48 and 72 h after ischemia. After the evaluation of neurologic function (48 h), the spinal cords were immediately removed for the determination of myeloperoxidase (MPO) activity (12–72 h). Apoptosis was quantitatively measured using the terminal transferase UTP nick end-labeling (TUNEL) method (24 h). The expression of bax and bcl-2 were evaluated by Western blot analysis (1 h), and GHSR-1a mRNA expression was detected using reverse transcriptase polymerase chain reaction (24 h). The neurological motor function was evaluated by ‘Tarlov’s score’. The neurologic outcomes in the ghrelin-group were significantly better than those in the vehicle group (p < 0.05). Serum tumor necrosis factor (TNF-α) levels were assessed in the peripheral venous blood. Ghrelin decreased the serum TNF-α levels and ameliorated the down regulation of spinal cord MPO activity. The expression of ghrelin receptors (GHSR-1a) in the rat spinal cord was decreased by I/R injury and increased by ghrelin. Ghrelin reduced the TUNEL-positive rate. Greater bcl-2, HSP27, HSP70, and attenuated bax expression were observed in the ghrelin-treated rats. Our results suggest that ghrelin administration may inhibit spinal I/R injury. Moreover, the improvement of neurologic function in rats was increased after the ghrelin treatment.
Keywords: ischemia/reperfusion injury; spinal cord; ghrelin ischemia/reperfusion injury; spinal cord; ghrelin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Zhang, Q.; Huang, C.; Meng, B.; Tang, T.; Shi, Q.; Yang, H. Acute Effect of Ghrelin on Ischemia/Reperfusion Injury in the Rat Spinal Cord. Int. J. Mol. Sci. 2012, 13, 9864-9876.

AMA Style

Zhang Q, Huang C, Meng B, Tang T, Shi Q, Yang H. Acute Effect of Ghrelin on Ischemia/Reperfusion Injury in the Rat Spinal Cord. International Journal of Molecular Sciences. 2012; 13(8):9864-9876.

Chicago/Turabian Style

Zhang, Qin; Huang, Chen; Meng, Bin; Tang, Tiansi; Shi, Qin; Yang, Huilin. 2012. "Acute Effect of Ghrelin on Ischemia/Reperfusion Injury in the Rat Spinal Cord." Int. J. Mol. Sci. 13, no. 8: 9864-9876.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert