Next Article in Journal
Expression Patterns, Activities and Carbohydrate-Metabolizing Regulation of Sucrose Phosphate Synthase, Sucrose Synthase and Neutral Invertase in Pineapple Fruit during Development and Ripening
Previous Article in Journal
Micelle and Bilayer Formation of Amphiphilic Janus Particles in a Slit-Pore
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(8), 9447-9459; doi:10.3390/ijms13089447

Pre-Ischemic Treadmill Training for Prevention of Ischemic Brain Injury via Regulation of Glutamate and Its Transporter GLT-1

Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China
State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China
The Third Teaching Hospital of Xinxiang Medical University, Xinxiang 453003, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 10 May 2012 / Revised: 14 July 2012 / Accepted: 19 July 2012 / Published: 26 July 2012
View Full-Text   |   Download PDF [230 KB, uploaded 19 June 2014]   |  


Pre-ischemic treadmill training exerts cerebral protection in the prevention of cerebral ischemia by alleviating neurotoxicity induced by excessive glutamate release following ischemic stroke. However, the underlying mechanism of this process remains unclear. Cerebral ischemia-reperfusion injury was observed in a rat model after 2 weeks of pre-ischemic treadmill training. Cerebrospinal fluid was collected using the microdialysis sampling method, and the concentration of glutamate was determined every 40 min from the beginning of ischemia to 4 h after reperfusion with high-performance liquid chromatography (HPLC)-fluorescence detection. At 3, 12, 24, and 48 h after ischemia, the expression of the glutamate transporter-1 (GLT-1) protein in brain tissues was determined by Western blot respectively. The effect of pre-ischemic treadmill training on glutamate concentration and GLT-1 expression after cerebral ischemia in rats along with changes in neurobehavioral score and cerebral infarct volume after 24 h ischemia yields critical information necessary to understand the protection mechanism exhibited by pre-ischemic treadmill training. The results demonstrated that pre-ischemic treadmill training up-regulates GLT-1 expression, decreases extracellular glutamate concentration, reduces cerebral infarct volume, and improves neurobehavioral score. Pre-ischemic treadmill training is likely to induce neuroprotection after cerebral ischemia by regulating GLT-1 expression, which results in re-uptake of excessive glutamate. View Full-Text
Keywords: glutamate; glutamate transporter-1 (GLT-1); pre-ischemic treadmill training; ischemia; neuroprotection glutamate; glutamate transporter-1 (GLT-1); pre-ischemic treadmill training; ischemia; neuroprotection

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yang, X.; He, Z.; Zhang, Q.; Wu, Y.; Hu, Y.; Wang, X.; Li, M.; Wu, Z.; Guo, Z.; Guo, J.; Jia, J. Pre-Ischemic Treadmill Training for Prevention of Ischemic Brain Injury via Regulation of Glutamate and Its Transporter GLT-1. Int. J. Mol. Sci. 2012, 13, 9447-9459.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top