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Int. J. Mol. Sci. 2012, 13(7), 9343-9362; doi:10.3390/ijms13079343
Article

Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, Oryza rufipogon Receptor-Like Protein Kinase 1

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Received: 30 May 2012; in revised form: 6 July 2012 / Accepted: 6 July 2012 / Published: 24 July 2012
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
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Abstract: Many of the plant leucine rich repeat receptor-like kinases (LRR-RLKs) have been found to regulate signaling during plant defense processes. In this study, we selected and sequenced an LRR-RLK gene, designated as Oryza rufipogon receptor-like protein kinase 1 (OrufRPK1), located within yield QTL yld1.1 from the wild rice Oryza rufipogon (accession IRGC105491). A 2055 bp coding region and two exons were identified. Southern blotting determined OrufRPK1 to be a single copy gene. Sequence comparison with cultivated rice orthologs (OsI219RPK1, OsI9311RPK1 and OsJNipponRPK1, respectively derived from O. sativa ssp. indica cv. MR219, O. sativa ssp. indica cv. 9311 and O. sativa ssp. japonica cv. Nipponbare) revealed the presence of 12 single nucleotide polymorphisms (SNPs) with five non-synonymous substitutions, and 23 insertion/deletion sites. The biological role of the OrufRPK1 as a defense related LRR-RLK is proposed on the basis of cDNA sequence characterization, domain subfamily classification, structural prediction of extra cellular domains, cluster analysis and comparative gene expression.
Keywords: Oryza rufipogon; RPK1; LRR domain; kinase domain; disease resistance Oryza rufipogon; RPK1; LRR domain; kinase domain; disease resistance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Law, Y.-S.; Gudimella, R.; Song, B.-K.; Ratnam, W.; Harikrishna, J.A. Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, Oryza rufipogon Receptor-Like Protein Kinase 1. Int. J. Mol. Sci. 2012, 13, 9343-9362.

AMA Style

Law Y-S, Gudimella R, Song B-K, Ratnam W, Harikrishna JA. Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, Oryza rufipogon Receptor-Like Protein Kinase 1. International Journal of Molecular Sciences. 2012; 13(7):9343-9362.

Chicago/Turabian Style

Law, Yee-Song; Gudimella, Ranganath; Song, Beng-Kah; Ratnam, Wickneswari; Harikrishna, Jennifer Ann. 2012. "Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, Oryza rufipogon Receptor-Like Protein Kinase 1." Int. J. Mol. Sci. 13, no. 7: 9343-9362.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert