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• Yue, W
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Int. J. Mol. Sci. 2012, 13(7), 9129-9141; doi:10.3390/ijms13079129

Article

Pharmacokinetics and Tissue Distribution Study of Praeruptorin D from Radix Peucedani in Rats by High-Performance Liquid Chromatography (HPLC)

Taigang Liang ^† , Wenyan Yue ^† , Xue Du, Luhui Ren and Qingshan Li*

School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 18 June 2012; in revised form: 16 July 2012 / Accepted: 17 July 2012 / Published: 20 July 2012

(This article belongs to the Section Bioactives and Nutraceuticals)

Download PDF Full-Text [218 KB, uploaded 20 July 2012 11:16 CEST]

Abstract: Praeruptorin D (PD), a major pyranocoumarin isolated from Radix Peucedani, exhibited antitumor and anti-inflammatory activities. The aim of this study was to investigate the pharmacokinetics and tissue distribution of PD in rats following intravenous (i.v.) administration. The levels of PD in plasma and tissues were measured by a simple and sensitive reversed-phase high-performance liquid chromatography (HPLC) method. The biosamples were treated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE) and osthole was used as the internal standard (IS). The chromatographic separation was accomplished on a reversed-phase C₁₈ column using methanol-water (75:25, v/v) as mobile phase at a flow rate of 0.8 mL/min and ultraviolet detection wave length was set at 323 nm. The results demonstrate that this method has excellent specificity, linearity, precision, accuracy and recovery. The pharmacokinetic study found that PD fitted well into a two-compartment model with a fast distribution phase and a relative slow elimination phase. Tissue distribution showed that the highest concentration was observed in the lung, followed by heart, liver and kidney. Furthermore, PD can also be detected in the brain, which indicated that PD could cross the blood-brain barrier after i.v. administration.

Keywords: praeruptorin D; Radix Peucedani; HPLC; pharmacokinetics; tissue distribution

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