Next Article in Journal
l-2-Oxothiazolidine-4-Carboxylic Acid or α-Lipoic Acid Attenuates Airway Remodeling: Involvement of Nuclear Factor-κB (NF-κB), Nuclear Factor Erythroid 2p45-Related Factor-2 (Nrf2), and Hypoxia-Inducible Factor (HIF)
Previous Article in Journal
Molecular Targets of TRAIL-Sensitizing Agents in Colorectal Cancer
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(7), 7902-7914; doi:10.3390/ijms13077902

A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways

1
Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China
2
Department of Periodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China
3
Department of Immunology, Medical College of Norman Bethune, Jilin University, Changchun 130021, China
4
Department of Molecular Biology, Medical College of Norman Bethune, Jilin University, Changchun 130021, China
*
Author to whom correspondence should be addressed.
Received: 10 May 2012 / Revised: 11 May 2012 / Accepted: 15 June 2012 / Published: 25 June 2012
View Full-Text   |   Download PDF [413 KB, uploaded 19 June 2014]   |  

Abstract

A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced the expression of osteogenic marker genes, such as osteocalcin and type I collagen. Furthermore, ODN MT01 activated Runx2 phosphorylation via ERK1/2 mitogen-activated protein kinase (MAPK) and p38 MAPK. Consistently, ODN MT01 induced up-regulation of osteocalcin, alkaline phosphatase (ALP) and type I collagen, which was inhibited by pre-treatment with the ERK1/2 inhibitor U0126 and the p38 inhibitor SB203580. These results suggest that the ERK1/2 and p38 MAPK pathways, as well as Runx2 activation, are involved in ODN MT01-induced up-regulation of osteocalcin, type I collagen and the activity of ALP in MG 63 cells.
Keywords: oligodeoxynucleotide; osteoblast; differentiation; ERK1/2 MAPK; p38 MAPK oligodeoxynucleotide; osteoblast; differentiation; ERK1/2 MAPK; p38 MAPK
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hou, X.; Shen, Y.; Zhang, C.; Zhang, L.; Qin, Y.; Yu, Y.; Wang, L.; Sun, X. A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways. Int. J. Mol. Sci. 2012, 13, 7902-7914.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top