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Int. J. Mol. Sci. 2012, 13(6), 7676-7693; doi:10.3390/ijms13067676
Article

Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

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Received: 27 March 2012; in revised form: 19 April 2012 / Accepted: 5 June 2012 / Published: 21 June 2012
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Abstract: Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient’s death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.
Keywords: multiple sclerosis; fulminant; shotgun-proteomics; protein quantification; isobaric tag labeling; matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF/TOF MS) multiple sclerosis; fulminant; shotgun-proteomics; protein quantification; isobaric tag labeling; matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF/TOF MS)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Füvesi, J.; Hanrieder, J.; Bencsik, K.; Rajda, C.; Kovács, S.K.; Kaizer, L.; Beniczky, S.; Vécsei, L.; Bergquist, J. Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis. Int. J. Mol. Sci. 2012, 13, 7676-7693.

AMA Style

Füvesi J, Hanrieder J, Bencsik K, Rajda C, Kovács SK, Kaizer L, Beniczky S, Vécsei L, Bergquist J. Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis. International Journal of Molecular Sciences. 2012; 13(6):7676-7693.

Chicago/Turabian Style

Füvesi, Judit; Hanrieder, Jörg; Bencsik, Krisztina; Rajda, Cecilia; Kovács, S. Krisztián; Kaizer, László; Beniczky, Sándor; Vécsei, László; Bergquist, Jonas. 2012. "Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis." Int. J. Mol. Sci. 13, no. 6: 7676-7693.



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