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Int. J. Mol. Sci. 2012, 13(6), 7676-7693; doi:10.3390/ijms13067676
Article

Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

1,2
, 2,3
, 1
, 1,4
, 5
, 5
, 1,4
, 1,6
 and 2,7,*
1 Department of Neurology, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged 6725, Hungary 2 Analytical Chemistry, Department of Chemistry-Biomedical Center, Uppsala University, P.O. Box 599, Uppsala 75124, Sweden 3 Analytical Chemistry, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Göteborg 41296, Sweden 4 Department of Clinical Neurophysiology, Danish Epilepsy Centre, Dianalund 4293, Denmark 5 Department of Pathology, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged 6725, Hungary 6 Neuroscience Research Group, Hungarian Academy of Sciences, University of Szeged, Semmelweis str 6, Szeged 6725, Hungary 7 Science for Life Laboratory (SciLife Lab), Uppsala University, P.O. Box 599, Uppsala 75124, Sweden
* Author to whom correspondence should be addressed.
Received: 27 March 2012 / Revised: 19 April 2012 / Accepted: 5 June 2012 / Published: 21 June 2012
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Abstract

Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient’s death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.
Keywords: multiple sclerosis; fulminant; shotgun-proteomics; protein quantification; isobaric tag labeling; matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF/TOF MS) multiple sclerosis; fulminant; shotgun-proteomics; protein quantification; isobaric tag labeling; matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF/TOF MS)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Füvesi, J.; Hanrieder, J.; Bencsik, K.; Rajda, C.; Kovács, S.K.; Kaizer, L.; Beniczky, S.; Vécsei, L.; Bergquist, J. Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis. Int. J. Mol. Sci. 2012, 13, 7676-7693.

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