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Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis
Department of Neurology, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged 6725, Hungary
Analytical Chemistry, Department of Chemistry-Biomedical Center, Uppsala University, P.O. Box 599, Uppsala 75124, Sweden
Analytical Chemistry, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Göteborg 41296, Sweden
Department of Clinical Neurophysiology, Danish Epilepsy Centre, Dianalund 4293, Denmark
Department of Pathology, Albert Szent-Györgyi Clinical Center, University of Szeged, Szeged 6725, Hungary
Neuroscience Research Group, Hungarian Academy of Sciences, University of Szeged, Semmelweis str 6, Szeged 6725, Hungary
Science for Life Laboratory (SciLife Lab), Uppsala University, P.O. Box 599, Uppsala 75124, Sweden
* Author to whom correspondence should be addressed.
Received: 27 March 2012; in revised form: 19 April 2012 / Accepted: 5 June 2012 / Published: 21 June 2012
Abstract: Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient’s death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.
Keywords: multiple sclerosis; fulminant; shotgun-proteomics; protein quantification; isobaric tag labeling; matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF/TOF MS)
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Füvesi, J.; Hanrieder, J.; Bencsik, K.; Rajda, C.; Kovács, S.K.; Kaizer, L.; Beniczky, S.; Vécsei, L.; Bergquist, J. Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis. Int. J. Mol. Sci. 2012, 13, 7676-7693.
Füvesi J, Hanrieder J, Bencsik K, Rajda C, Kovács SK, Kaizer L, Beniczky S, Vécsei L, Bergquist J. Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis. International Journal of Molecular Sciences. 2012; 13(6):7676-7693.
Füvesi, Judit; Hanrieder, Jörg; Bencsik, Krisztina; Rajda, Cecilia; Kovács, S. Krisztián; Kaizer, László; Beniczky, Sándor; Vécsei, László; Bergquist, Jonas. 2012. "Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis." Int. J. Mol. Sci. 13, no. 6: 7676-7693.