Abstract: In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE) of syringin from the bark of Ilex rotunda Thumb using response surface methodology (RSM) is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD) to obtain the highest extraction efficiency. The optimal conditions were the use of a sonication frequency of 40 kHz, 65% methanol as the solvent, an extraction time of 30 min and an extraction temperature of 40 °C. Using these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing syringin extraction from the bark of I. rotunda.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Zhao, L.-C.; He, Y.; Deng, X.; Xia, X.-H.; Liang, J.; Yang, G.-L.; Li, W.; Wang, H. Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 7607-7616.
Zhao L-C, He Y, Deng X, Xia X-H, Liang J, Yang G-L, Li W, Wang H. Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology. International Journal of Molecular Sciences. 2012; 13(6):7607-7616.
Zhao, Li-Chun; He, Ying; Deng, Xin; Xia, Xiang-Hua; Liang, Jian; Yang, Geng-Liang; Li, Wei; Wang, Hui. 2012. "Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology." Int. J. Mol. Sci. 13, no. 6: 7607-7616.