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Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology
College of Pharmacy, Hebei University, Baoding 071002, China
The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
China-Japan Union Hospital, Jilin University, Changchun 130033, China
* Authors to whom correspondence should be addressed.
Received: 28 April 2012; in revised form: 12 May 2012 / Accepted: 15 May 2012 / Published: 20 June 2012
Abstract: In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE) of syringin from the bark of Ilex rotunda Thumb using response surface methodology (RSM) is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD) to obtain the highest extraction efficiency. The optimal conditions were the use of a sonication frequency of 40 kHz, 65% methanol as the solvent, an extraction time of 30 min and an extraction temperature of 40 °C. Using these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing syringin extraction from the bark of I. rotunda.
Keywords: ultrasound-assisted extraction; syringin; Ilex rotunda; response surface methodology; Box-Behnken design
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MDPI and ACS Style
Zhao, L.-C.; He, Y.; Deng, X.; Xia, X.-H.; Liang, J.; Yang, G.-L.; Li, W.; Wang, H. Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 7607-7616.
Zhao L-C, He Y, Deng X, Xia X-H, Liang J, Yang G-L, Li W, Wang H. Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology. International Journal of Molecular Sciences. 2012; 13(6):7607-7616.
Zhao, Li-Chun; He, Ying; Deng, Xin; Xia, Xiang-Hua; Liang, Jian; Yang, Geng-Liang; Li, Wei; Wang, Hui. 2012. "Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology." Int. J. Mol. Sci. 13, no. 6: 7607-7616.