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Int. J. Mol. Sci. 2012, 13(6), 7496-7507; doi:10.3390/ijms13067496

Simulated Gastrointestinal pH Condition Improves Antioxidant Properties of Wheat and Rice Flours

1
Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
2
Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan
*
Author to whom correspondence should be addressed.
Received: 16 April 2012 / Revised: 31 May 2012 / Accepted: 1 June 2012 / Published: 18 June 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The present study was conducted to evaluate the antioxidant properties of wheat and rice flours under simulated gastrointestinal pH condition. After subjecting the wheat and rice flour slurries to simulated gastrointestinal pH condition, both slurries were centrifuged to obtain the crude phenolic extracts for further analyses. Extraction yield, total contents of phenolic and flavonoids were determined as such (untreated) and under simulated gastrointestinal pH condition (treated). 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical cation (ABTS•+) scavenging activity, ferric reducing antioxidant power (FRAP), beta-carotene bleaching (BCB) and iron chelating activity assays were employed for the determination of antioxidant activity of the tested samples. In almost all of the assays performed, significant improvements in antioxidant properties (p < 0.05) were observed in both flours after treatment, suggesting that wheat and rice flours contain considerably heavy amounts of bound phenolics, and that their antioxidant properties might be improved under gastrointestinal digestive conditions.
Keywords: wheat flour; rice flour; antioxidant activity; phenolic contents; simulated gastrointestinal pH wheat flour; rice flour; antioxidant activity; phenolic contents; simulated gastrointestinal pH
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chan, K.W.; Khong, N.M.H.; Iqbal, S.; Ismail, M. Simulated Gastrointestinal pH Condition Improves Antioxidant Properties of Wheat and Rice Flours. Int. J. Mol. Sci. 2012, 13, 7496-7507.

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