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Int. J. Mol. Sci. 2012, 13(5), 6320-6333; doi:10.3390/ijms13056320

Anti-Hyperglycemic Effect of Chebulagic Acid from the Fruits of Terminalia chebula Retz

3,*  and 3,*
1 Department of Public Health, Hua Xi Medicinal Center of Sichuan University, Chengdu 610041, China 2 Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital, Chengdu 610072, China 3 College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 1 April 2012 / Revised: 14 May 2012 / Accepted: 16 May 2012 / Published: 22 May 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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In the present study, we firstly compared rat intestinal α-glucosidase inhibitory activity by different ethanol-aqueous extractions from the dried fruits of Terminalia chebula Retz. The enzymatic assay showed that the 80% ethanol extract was more potent against maltase activity than both 50% and 100% ethanol extracts. By HPLC analysis, it was determined that the 80% ethanol extract had a higher content of chebulagic acid than each of 50% or 100% ethanol extract. Next, we investigated how efficiently chebulagic acid could inhibit sugar digestion by determining the glucose level on the apical side of the Caco-2 cell monolayer. The result showed that the maltose-hydrolysis activity was down-regulated by chebulagic acid, which proved to be a reversible inhibitor of maltase in Caco-2 cells. On the other hand, chebulagic acid showed a weak inhibition of sucrose-hydrolysis activity. Meanwhile, chebulagic acid did not have an obvious influence on intestinal glucose uptake and was not effective on glucose transporters. Further animal studies revealed that the oral administration of chebulagic acid (100 mg/kg body weight) significantly reduced postprandial blood glucose levels by 11.1% in maltose-loaded Sprague-Dawley (SD) rats compared with the control group, whereas the oral administration of chebulagic acid did not show a suppressive effect on postprandial hyperglycemia in sucrose- or glucose-loaded SD-rats. The results presented here suggest that chebulagic acid from T. chebula can be used to control blood glucose and manage type 2 diabetes, although clinical trials are needed.
Keywords: Terminalia chebula; chebulagic acid; α-glucosidase inhibitor; anti-hyperglycemia Terminalia chebula; chebulagic acid; α-glucosidase inhibitor; anti-hyperglycemia
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, Y.-N.; Zhao, D.-D.; Gao, B.; Zhong, K.; Zhu, R.-X.; Zhang, Y.; Xie, W.-J.; Jia, L.-R.; Gao, H. Anti-Hyperglycemic Effect of Chebulagic Acid from the Fruits of Terminalia chebula Retz. Int. J. Mol. Sci. 2012, 13, 6320-6333.

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