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Int. J. Mol. Sci. 2012, 13(5), 6220-6235; doi:10.3390/ijms13056220
Article

Antioxidative Characteristics of Anisomeles indica Extract and Inhibitory Effect of Ovatodiolide on Melanogenesis

1
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2
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3
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3
,
4
 and
3,*
1 Department of Medical Laboratory Science and Biotechnology, China Medical University, No, 91 Hsueh-Shih Road, Taichung 40402, Taiwan 2 Department of Chemistry, Tung Hai University, No, 181, Section 3, Taichung Port Road, Taichung 40704, Taiwan 3 Department of Applied Cosmetology & Master Program of Cosmetic Science, Hung Kuang University, No. 34, Chung-Chie Rd., Shalu, Taichung 43302, Taiwan 4 School of Management Department, Feng Chia University, No, 100, Wenhwa Road, Seatwen, Taichung 40724, Taiwan
* Author to whom correspondence should be addressed.
Received: 9 April 2012 / Revised: 10 May 2012 / Accepted: 14 May 2012 / Published: 21 May 2012
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Abstract

The purpose of the study was to investigate the antioxidant characteristics of Anisomeles indica methanol extract and the inhibitory effect of ovatodiolide on melanogenesis. In the study, the antioxidant capacities of A. indica methanol extract such as DPPH assay, ABTS radical scavenging assay, reducing capacity and metal ion chelating capacity as well as total phenolic content of the extract were investigated. In addition, the inhibitory effects of ovatodiolide on mushroom tyrosinase, B16F10 intracellular tyrosinase and melanin content were determined spectrophotometrically. Our results revealed that the antioxidant capacities of A. indica methanol extract increased in a dose-dependent pattern. The purified ovatodiolide inhibited mushroom tyrosinase activity (IC50 = 0.253 mM), the compound also effectively suppressed intracellular tyrosinase activity (IC50 = 0.469 mM) and decreased the amount of melanin (IC50 = 0.435 mM) in a dose-dependent manner in B16F10 cells. Our results concluded that A. indica methanol extract displays antioxidant capacities and ovatodiolide purified from the extract inhibited melanogenesis in B16F10 cells. Hence, A. indica methanol extract and ovatodiolide could be applied as a type of dermatological whitening agent in skin care products.
Keywords: Anisomeles indica; ovatodiolide; melanogenesis; tyrosinase; melanin; antioxidant Anisomeles indica; ovatodiolide; melanogenesis; tyrosinase; melanin; antioxidant
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, H.-C.; Lien, H.-M.; Ke, H.-J.; Chang, L.-L.; Chen, C.-C.; Chang, T.-M. Antioxidative Characteristics of Anisomeles indica Extract and Inhibitory Effect of Ovatodiolide on Melanogenesis. Int. J. Mol. Sci. 2012, 13, 6220-6235.

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