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Expression of Selected Ginkgo biloba Heat Shock Protein Genes After Cold Treatment Could Be Induced by Other Abiotic Stress
Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, China
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
College of Chemistry and life science, Huanggang Normal University, Huanggang 438000, China
* Authors to whom correspondence should be addressed.
Received: 21 March 2012; in revised form: 26 April 2012 / Accepted: 2 May 2012 / Published: 15 May 2012
Abstract: Heat shock proteins (HSPs) play various stress-protective roles in plants. In this study, three HSP genes were isolated from a suppression subtractive hybridization (SSH) cDNA library of Ginkgo biloba leaves treated with cold stress. Based on the molecular weight, the three genes were designated GbHSP16.8, GbHSP17 and GbHSP70. The full length of the three genes were predicted to encode three polypeptide chains containing 149 amino acids (Aa), 152 Aa, and 657 Aa, and their corresponding molecular weights were predicted as follows: 16.67 kDa, 17.39 kDa, and 71.81 kDa respectively. The three genes exhibited distinctive expression patterns in different organs or development stages. GbHSP16.8 and GbHSP70 showed high expression levels in leaves and a low level in gynoecia, GbHSP17 showed a higher transcription in stamens and lower level in fruit. This result indicates that GbHSP16.8 and GbHSP70 may play important roles in Ginkgo leaf development and photosynthesis, and GbHSP17 may play a positive role in pollen maturation. All three GbHSPs were up-regulated under cold stress, whereas extreme heat stress only caused up-regulation of GbHSP70, UV-B treatment resulted in up-regulation of GbHSP16.8 and GbHSP17, wounding treatment resulted in up-regulation of GbHSP16.8 and GbHSP70, and abscisic acid (ABA) treatment caused up-regulation of GbHSP70 primarily.
Keywords: heat shock proteins; SSH; cold stress; heat stress; abiotic stress; Ginkgo biloba
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MDPI and ACS Style
Cao, F.; Cheng, H.; Cheng, S.; Li, L.; Xu, F.; Yu, W.; Yuan, H. Expression of Selected Ginkgo biloba Heat Shock Protein Genes After Cold Treatment Could Be Induced by Other Abiotic Stress. Int. J. Mol. Sci. 2012, 13, 5768-5788.
Cao F, Cheng H, Cheng S, Li L, Xu F, Yu W, Yuan H. Expression of Selected Ginkgo biloba Heat Shock Protein Genes After Cold Treatment Could Be Induced by Other Abiotic Stress. International Journal of Molecular Sciences. 2012; 13(5):5768-5788.
Cao, Fuliang; Cheng, Hua; Cheng, Shuiyuan; Li, Linling; Xu, Feng; Yu, Wanwen; Yuan, Honghui. 2012. "Expression of Selected Ginkgo biloba Heat Shock Protein Genes After Cold Treatment Could Be Induced by Other Abiotic Stress." Int. J. Mol. Sci. 13, no. 5: 5768-5788.