Int. J. Mol. Sci. 2012, 13(5), 5700-5705; doi:10.3390/ijms13055700
Development of Nine Markers and Characterization of the Microsatellite Loci in the Endangered Gymnogobius isaza (Gobiidae)
Center for Ecological Research, Kyoto University, Hirano 2-509-3, Otsu 520-2113, Japan
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Received: 5 April 2012 / Revised: 28 April 2012 / Accepted: 7 May 2012 / Published: 11 May 2012
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract
Gymnogobius isaza is a freshwater goby endemic to Lake Biwa, Japan. They experienced a drastic demographic bottleneck in the 1950s and 1980s and slightly recovered thereafter, but the population size is still very small. To reveal dynamics of genetic diversity of G. isaza, we developed nine microsatellite markers based on the sequence data of a related goby Chaenogobius annularis. Nine SSR (Simple Sequence Repeats) markers were successfully amplified for raw and formalin-fixed fish samples. The number of alleles and expected heterozygosities ranged from one to 10 and from 0.06 to 0.84, respectively, for the current samples, while one to 12 and 0.09 to 0.83 for historical samples. The markers described here will be useful for investigating the genetic diversity and gene flow and for conservation of G. isaza. View Full-TextKeywords:
bottleneck; formalin-fixed samples; Gymnogobius isaza; Lake Biwa; microsatellite
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Araki, K.S.; Nakazawa, T.; Kawakita, A.; Kudoh, H.; Okuda, N. Development of Nine Markers and Characterization of the Microsatellite Loci in the Endangered Gymnogobius isaza (Gobiidae). Int. J. Mol. Sci. 2012, 13, 5700-5705.
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