Int. J. Mol. Sci. 2012, 13(4), 5254-5277; doi:10.3390/ijms13045254
Review

Phage Displayed Peptides/Antibodies Recognizing Growth Factors and Their Tyrosine Kinase Receptors as Tools for Anti-Cancer Therapeutics

Roberto Ronca 1 email, Patrizia Benzoni 2 email, Angela De Luca 2 email, Elisabetta Crescini 2 email and Patrizia Dell’Era 2,* email
1 Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy 2 Fibroblast Reprogramming Unit, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy
* Author to whom correspondence should be addressed.
Received: 9 February 2012; in revised form: 9 April 2012 / Accepted: 20 April 2012 / Published: 24 April 2012
(This article belongs to the Special Issue Phage Display)
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Abstract: The basic idea of displaying peptides on a phage, introduced by George P. Smith in 1985, was greatly developed and improved by McCafferty and colleagues at the MRC Laboratory of Molecular Biology and, later, by Barbas and colleagues at the Scripps Research Institute. Their approach was dedicated to building a system for the production of antibodies, similar to a naïve B cell repertoire, in order to by-pass the standard hybridoma technology that requires animal immunization. Both groups merged the phage display technology with an antibody library to obtain a huge number of phage variants, each of them carrying a specific antibody ready to bind its target molecule, allowing, later on, rare phage (one in a million) to be isolated by affinity chromatography. Here, we will briefly review the basis of the technology and the therapeutic application of phage-derived bioactive molecules when addressed against key players in tumor development and progression: growth factors and their tyrosine kinase receptors.
Keywords: phage display; humanized antibody; growth factors; tyrosine kinase receptors; anticancer therapy

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MDPI and ACS Style

Ronca, R.; Benzoni, P.; De Luca, A.; Crescini, E.; Dell’Era, P. Phage Displayed Peptides/Antibodies Recognizing Growth Factors and Their Tyrosine Kinase Receptors as Tools for Anti-Cancer Therapeutics. Int. J. Mol. Sci. 2012, 13, 5254-5277.

AMA Style

Ronca R, Benzoni P, De Luca A, Crescini E, Dell’Era P. Phage Displayed Peptides/Antibodies Recognizing Growth Factors and Their Tyrosine Kinase Receptors as Tools for Anti-Cancer Therapeutics. International Journal of Molecular Sciences. 2012; 13(4):5254-5277.

Chicago/Turabian Style

Ronca, Roberto; Benzoni, Patrizia; De Luca, Angela; Crescini, Elisabetta; Dell’Era, Patrizia. 2012. "Phage Displayed Peptides/Antibodies Recognizing Growth Factors and Their Tyrosine Kinase Receptors as Tools for Anti-Cancer Therapeutics." Int. J. Mol. Sci. 13, no. 4: 5254-5277.

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