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Int. J. Mol. Sci. 2012, 13(4), 4889-4895; doi:10.3390/ijms13044889

Development of Eighteen Microsatellite Markers in Anemone amurensis (Ranunculaceae) and Cross-Amplification in Congeneric Species

Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China
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Received: 10 February 2012 / Revised: 11 April 2012 / Accepted: 11 April 2012 / Published: 18 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Polyploidy plays an important role in the evolution of plant genomes. To enable the investigation of the polyploidy events within the genus Anemone, we developed eighteen microsatellite markers from the hexaploid species A. amurensis (Ranunculaceae), and tested their transferability in five closely related species. The number of total alleles (NA) for each resulting locus varied from one to eight. The polymorphism information content (PIC) and Nei’s genetic diversity (NGD) for these microsatellites ranged from 0.00 to 0.71 and 0.00 to 0.91, respectively. For each population, the NA was one to seven, and the values of PIC and NGD varied from 0.00 to 0.84 and 0.00 to 0.95, respectively. In addition, most of these microsatellites can be amplified successfully in the congeneric species. These microsatellite primers provide us an opportunity to study the polyploid evolution in the genus Anemone.
Keywords: Anemone; A. amurensis; microsatellite; polyploidy; polymorphism information content Anemone; A. amurensis; microsatellite; polyploidy; polymorphism information content
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sun, M.; Yin, X.; Shi, F.; Li, L.; Li, M.; Li, L.; Xiao, H. Development of Eighteen Microsatellite Markers in Anemone amurensis (Ranunculaceae) and Cross-Amplification in Congeneric Species. Int. J. Mol. Sci. 2012, 13, 4889-4895.

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