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Int. J. Mol. Sci. 2012, 13(4), 4873-4879; doi:10.3390/ijms13044873
Article

Study of Drug Metabolism by Xanthine Oxidase

1
,
1
,
2
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2,*  and 1,3,*
1 Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China 2 Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China 3 Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
* Authors to whom correspondence should be addressed.
Received: 13 January 2012 / Revised: 31 March 2012 / Accepted: 16 April 2012 / Published: 18 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

In this work, we report the studies of drug metabolism by xanthine oxidase (XOD) with electrochemical techniques. Firstly, a pair of stable, well-defined and quasi-reversible oxidation/reduction peaks is obtained with the formal potential at −413.1 mV (vs. SCE) after embedding XOD in salmon sperm DNA membrane on the surface of pyrolytic graphite electrode. Then, a new steady peak can be observed at −730 mV (vs. SCE) upon the addition of 6-mercaptopurine (6-MP) to the electrochemical system, indicating the metabolism of 6-MP by XOD. Furthermore, the chronoamperometric response shows that the current of the catalytic peak located at −730 mV increases with addition of 6-MP in a concentration-dependent manner, and the increase of the chronoamperometric current can be inhibited by an XOD inhibitor, quercetin. Therefore, our results prove that XOD/DNA modified electrode can be efficiently used to study the metabolism of 6-MP, which may provide a convenient approach for in vitro studies on enzyme-catalyzed drug metabolism.
Keywords: xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Zhao, J.; He, X.; Yang, N.; Sun, L.; Li, G. Study of Drug Metabolism by Xanthine Oxidase. Int. J. Mol. Sci. 2012, 13, 4873-4879.

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