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Int. J. Mol. Sci. 2012, 13(4), 4873-4879; doi:10.3390/ijms13044873

Study of Drug Metabolism by Xanthine Oxidase

Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
Authors to whom correspondence should be addressed.
Received: 13 January 2012 / Revised: 31 March 2012 / Accepted: 16 April 2012 / Published: 18 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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In this work, we report the studies of drug metabolism by xanthine oxidase (XOD) with electrochemical techniques. Firstly, a pair of stable, well-defined and quasi-reversible oxidation/reduction peaks is obtained with the formal potential at −413.1 mV (vs. SCE) after embedding XOD in salmon sperm DNA membrane on the surface of pyrolytic graphite electrode. Then, a new steady peak can be observed at −730 mV (vs. SCE) upon the addition of 6-mercaptopurine (6-MP) to the electrochemical system, indicating the metabolism of 6-MP by XOD. Furthermore, the chronoamperometric response shows that the current of the catalytic peak located at −730 mV increases with addition of 6-MP in a concentration-dependent manner, and the increase of the chronoamperometric current can be inhibited by an XOD inhibitor, quercetin. Therefore, our results prove that XOD/DNA modified electrode can be efficiently used to study the metabolism of 6-MP, which may provide a convenient approach for in vitro studies on enzyme-catalyzed drug metabolism.
Keywords: xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhao, J.; He, X.; Yang, N.; Sun, L.; Li, G. Study of Drug Metabolism by Xanthine Oxidase. Int. J. Mol. Sci. 2012, 13, 4873-4879.

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