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Study of Drug Metabolism by Xanthine Oxidase
Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
* Authors to whom correspondence should be addressed.
Received: 13 January 2012; in revised form: 31 March 2012 / Accepted: 16 April 2012 / Published: 18 April 2012
Abstract: In this work, we report the studies of drug metabolism by xanthine oxidase (XOD) with electrochemical techniques. Firstly, a pair of stable, well-defined and quasi-reversible oxidation/reduction peaks is obtained with the formal potential at −413.1 mV (vs. SCE) after embedding XOD in salmon sperm DNA membrane on the surface of pyrolytic graphite electrode. Then, a new steady peak can be observed at −730 mV (vs. SCE) upon the addition of 6-mercaptopurine (6-MP) to the electrochemical system, indicating the metabolism of 6-MP by XOD. Furthermore, the chronoamperometric response shows that the current of the catalytic peak located at −730 mV increases with addition of 6-MP in a concentration-dependent manner, and the increase of the chronoamperometric current can be inhibited by an XOD inhibitor, quercetin. Therefore, our results prove that XOD/DNA modified electrode can be efficiently used to study the metabolism of 6-MP, which may provide a convenient approach for in vitro studies on enzyme-catalyzed drug metabolism.
Keywords: xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin
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MDPI and ACS Style
Zhao, J.; He, X.; Yang, N.; Sun, L.; Li, G. Study of Drug Metabolism by Xanthine Oxidase. Int. J. Mol. Sci. 2012, 13, 4873-4879.
Zhao J, He X, Yang N, Sun L, Li G. Study of Drug Metabolism by Xanthine Oxidase. International Journal of Molecular Sciences. 2012; 13(4):4873-4879.
Zhao, Jing; He, Xiaolin; Yang, Nana; Sun, Lizhou; Li, Genxi. 2012. "Study of Drug Metabolism by Xanthine Oxidase." Int. J. Mol. Sci. 13, no. 4: 4873-4879.