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Int. J. Mol. Sci. 2012, 13(4), 4642-4654; doi:10.3390/ijms13044642

Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus

1,* , 1
Received: 20 January 2012 / Revised: 23 March 2012 / Accepted: 31 March 2012 / Published: 12 April 2012
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The objective of this study was to investigate the salivary proteins that are associated with periodontitis in patients with Type 2 diabetes mellitus (T2DM). Volunteers for the study were patients from the Diabetic Unit, University of Malaya Medical Centre, whose periodontal status was determined. The diabetic volunteers were divided into two groups, i.e., patients with periodontitis and those who were periodontally healthy. Saliva samples were collected and treated with 10% TCA/acetone/20 mM DTT to precipitate the proteins, which were then separated using two-dimensional polyacrylamide gel electrophoresis. Gel images were scanned using the GS-800TM Calibrated Densitometer. The protein spots were analyzed and expressed in percentage volumes. The percentage volume of each protein spot was subjected to Mann-Whitney statistical analysis using SPSS software and false discovery rate correction. When the expression of the salivary proteins was compared between the T2DM patients with periodontitis with those who were periodontally healthy, seven proteins, including polymeric immunoglobulin receptor, plastin-2, actin related protein 3, leukocyte elastase inhibitor, carbonic anhydrases 6, immunoglobulin J and interleukin-1 receptor antagonist, were found to be differentially expressed (p < 0.01304). This implies that the proteins may have the potential to be used as biomarkers for the prediction of T2DM patients who may be prone to periodontitis.
Keywords: periodontitis; type 2 diabetes mellitus; salivary protein; 2-DE periodontitis; type 2 diabetes mellitus; salivary protein; 2-DE
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chan, H.H.; Rahim, Z.H.A.; Jessie, K.; Hashim, O.H.; Taiyeb-Ali, T.B. Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus. Int. J. Mol. Sci. 2012, 13, 4642-4654.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert