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Int. J. Mol. Sci. 2012, 13(3), 2650-2675; doi:10.3390/ijms13032650
Article

Increased Activity of Cell Surface Peptidases in HeLa Cells Undergoing UV-Induced Apoptosis Is Not Mediated by Caspase 3

1,2,* , 2
, 2
, 2
 and 2
1 School of Medical Sciences, RMIT University, Bundoora 3083, Victoria, Australia 2 QCF Cancer Research Unit, Queensland Institute of Medical Research, Herston, Queensland 4006, Australia
* Author to whom correspondence should be addressed.
Received: 6 December 2011 / Revised: 14 February 2012 / Accepted: 22 February 2012 / Published: 28 February 2012
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

We have previously shown that in HeLa cells treated with a variety of agents there is an increase in cell surface peptidase (CSP) activity in those cells undergoing apoptosis. The increase in CSP activity observed in UVB-irradiated cells undergoing apoptosis was unaffected when the cultures were treated with the aminopeptidase inhibitor bestatin, and matrix metalloprotease inhibitor BB3103, but greatly enhanced when treated with the caspase 3 inhibitor-DEVD, and reduced in the presence of the poly(ADP-ribose) polymerase (PARP) inhibitor-3-aminobenzamide (3AB). Neither 3AB nor DEVD had an effect on the gross morphology of the apoptotic cells observed under electron microscopy, nor did they have an effect on phosphatidylserine eversion on the cell membrane, or that of PARP cleavage. All the agents except for DEVD had no effect on the level of caspase 3 activity in the cells. The results suggest that other caspases may cleave PARP in these cells. Both 3AB and DEVD treatment reduced the level of actin cleavage seen in the apoptotic cells. The increase in CSP activity observed in cells undergoing UVB-induced apoptosis appears to involve PARP but not caspase 3.
Keywords: cell surface peptidase; UV radiation; cell death; apoptosis cell surface peptidase; UV radiation; cell death; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Piva, T.J.; Davern, C.M.; Hall, P.M.; Winterford, C.M.; Ellem, K.A.O. Increased Activity of Cell Surface Peptidases in HeLa Cells Undergoing UV-Induced Apoptosis Is Not Mediated by Caspase 3. Int. J. Mol. Sci. 2012, 13, 2650-2675.

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