Int. J. Mol. Sci. 2012, 13(12), 17104-17120; doi:10.3390/ijms131217104
Article

High Density Lipoprotein Protects Mesenchymal Stem Cells from Oxidative Stress-Induced Apoptosis via Activation of the PI3K/Akt Pathway and Suppression of Reactive Oxygen Species

1 Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China 2 Institutes of Biomedical Scienses, Fudan University, Shanghai 200032, China 3 Department of Cardiology, Minhang Hospital, Ruijin Hospital Group, Shanghai Jiaotong University School of Medicine, Shanghai 201199, China
* Authors to whom correspondence should be addressed.
Received: 26 September 2012; in revised form: 11 November 2012 / Accepted: 4 December 2012 / Published: 13 December 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The therapeutic effect of transplantation of mesenchymal stem cells (MSCs) in myocardial infarction (MI) appears to be limited by poor cell viability in the injured tissue, which is a consequence of oxidative stress and pro-apoptotic factors. High density lipoprotein (HDL) reverses cholesterol transport and has anti-oxidative and anti-apoptotic properties. We, therefore, investigated whether HDL could protect MSCs from oxidative stress-induced apoptosis. MSCs derived from the bone marrow of rats were pre-incubated with or without HDL, and then were exposed to hydrogen peroxide (H2O2) in vitro, or were transplanted into experimentally infarcted hearts of rats in vivo. Pre-incubation of MSCs with HDL increased cell viability, reduced apoptotic indices and resulted in parallel decreases in reactive oxygen species (ROS) in comparison with control MSCs. Each of the beneficial effects of HDL on MSCs was attenuated by inhibiting the PI3K/Akt pathway. Preconditioning with HDL resulted in higher MSC survival rates, improved cardiac remodeling and better myocardial function than in the MSC control group. Collectively, these results suggest that HDL may protect against H2O2-induced apoptosis in MSCs through activation of a PI3K/Akt pathway, and by suppressing the production of ROS.
Keywords: high density lipoprotein; mesenchymal stem cells; oxidative stress; apoptosis; myocardial infarction

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MDPI and ACS Style

Xu, J.; Qian, J.; Xie, X.; Lin, L.; Zou, Y.; Fu, M.; Huang, Z.; Zhang, G.; Su, Y.; Ge, J. High Density Lipoprotein Protects Mesenchymal Stem Cells from Oxidative Stress-Induced Apoptosis via Activation of the PI3K/Akt Pathway and Suppression of Reactive Oxygen Species. Int. J. Mol. Sci. 2012, 13, 17104-17120.

AMA Style

Xu J, Qian J, Xie X, Lin L, Zou Y, Fu M, Huang Z, Zhang G, Su Y, Ge J. High Density Lipoprotein Protects Mesenchymal Stem Cells from Oxidative Stress-Induced Apoptosis via Activation of the PI3K/Akt Pathway and Suppression of Reactive Oxygen Species. International Journal of Molecular Sciences. 2012; 13(12):17104-17120.

Chicago/Turabian Style

Xu, Jianfeng; Qian, Juying; Xie, Xinxing; Lin, Li; Zou, Yunzeng; Fu, Mingqiang; Huang, Zheyong; Zhang, Guoping; Su, Yangang; Ge, Junbo. 2012. "High Density Lipoprotein Protects Mesenchymal Stem Cells from Oxidative Stress-Induced Apoptosis via Activation of the PI3K/Akt Pathway and Suppression of Reactive Oxygen Species." Int. J. Mol. Sci. 13, no. 12: 17104-17120.

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