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Int. J. Mol. Sci. 2012, 13(12), 17065-17076; doi:10.3390/ijms131217065
Article

Plant Regeneration and Somatic Embryogenesis from Immature Embryos Derived through Interspecific Hybridization among Different Carica Species

1,2,* , 3
 and 1
Received: 5 September 2012; in revised form: 15 November 2012 / Accepted: 4 December 2012 / Published: 12 December 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Plant regeneration and somatic embryogenesis through interspecific hybridization among different Carica species were studied for the development of a papaya ringspot virus-resistant variety. The maximum fruit sets were recorded from the cross of the native variety C. papaya cv. Shahi with the wild species C. cauliflora. The highest hybrid embryos were recorded at 90 days after pollination and the embryos were aborted at 150 days after pollination. The immature hybrid embryos were used for plant regeneration and somatic embryogenesis. The 90-day-old hybrid embryos from the cross of C. papaya cv. Shahi × C. cauliflora showed the highest percentage of germination, as well as plant regeneration on growth regulators free culture medium after 7 days pre-incubation on half-strength MS medium supplemented with 0.2 mg/L BAP, 0.5 mg/L NAA and 60 g/L sucrose. The 90-day-old hybrid embryos from the cross of C. papaya cv. Shahi × C. cauliflora produced maximum callus, as well as somatic embryos when cultured on half-strength MS medium containing 5 mg/L 2,4-D, 100 mg/L glutamine, 100 mg/L casein hydrolysate and 60 g/L sucrose. The somatic embryos were transferred into half-strength MS medium containing 0.5 mg/L BAP and 0.2 mg/L NAA and 60 g/L sucrose for maturation. The highest number of regenerated plants per hybrid embryo (10.33) was recorded from the cross of C. papaya cv. Shahi × C. cauliflora. Isoenzyme and dendrogram cluster analysis using UPGMA of the regenerated F1 plantlets confirmed the presence of the hybrid plantlets.
Keywords: Carica species; hybridization; plant regeneration; somatic embryogenesis Carica species; hybridization; plant regeneration; somatic embryogenesis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Azad, M.A.K.; Rabbani, M.G.; Amin, L. Plant Regeneration and Somatic Embryogenesis from Immature Embryos Derived through Interspecific Hybridization among Different Carica Species. Int. J. Mol. Sci. 2012, 13, 17065-17076.

AMA Style

Azad MAK, Rabbani MG, Amin L. Plant Regeneration and Somatic Embryogenesis from Immature Embryos Derived through Interspecific Hybridization among Different Carica Species. International Journal of Molecular Sciences. 2012; 13(12):17065-17076.

Chicago/Turabian Style

Azad, Md. Abul K.; Rabbani, Md. Golam; Amin, Latifah. 2012. "Plant Regeneration and Somatic Embryogenesis from Immature Embryos Derived through Interspecific Hybridization among Different Carica Species." Int. J. Mol. Sci. 13, no. 12: 17065-17076.


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