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Int. J. Mol. Sci. 2012, 13(12), 15881-15900; doi:10.3390/ijms131215881

Modulation of Adipogenic Conditions for Prospective Use of hADSCs in Adipose Tissue Engineering

Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95, Splaiul Independentei, sect. 5, Bucharest 050095, Romania These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 3 August 2012 / Revised: 15 October 2012 / Accepted: 8 November 2012 / Published: 27 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Modern strategies in adipose tissue engineering (ATE) take advantage of the easy harvest, abundance and differentiation potential towards mesenchymal lineages of hADSCs. The controlled conversion of hADSCs to committed adipogenic precursors and further mature adipocytes formation is important for good long-term results in soft tissue regeneration. Thus, in this study, we report: (i) the isolation of the processed lipoaspirate (PLA) cells from adipose tissue and sanguine fractions; (ii) the phenotypic characterization of the PLA descendants; (iii) the design of a novel protocol for the modulation of adipogenic conditions in the perspectives of ATE applications. To modulate the differentiation rate through our protocol, we propose to selectively modify the formulation of the adipogenic media in accordance with the evolution of the process. Therefore, we aimed to ensure the long-term proliferation of the precursor cells and to delay the late adipogenic events. The status of differentiation was characterized in terms of intracellular lipid accumulation and reorganization of the cytoskeleton simultaneously with perilipin protein expression. Moreover, we studied the sequential activation of PPARγ2, FAS, aP2 and perilipin genes which influence the kinetics of the adipogenic process. The strategies developed in this work are the prerequisites for prospective 3D regenerative systems.
Keywords: lipoaspirate; hADSC; adipogenesis; PPARγ2; perilipin; aP2; FAS; Oil Red O lipoaspirate; hADSC; adipogenesis; PPARγ2; perilipin; aP2; FAS; Oil Red O
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Galateanu, B.; Dinescu, S.; Cimpean, A.; Dinischiotu, A.; Costache, M. Modulation of Adipogenic Conditions for Prospective Use of hADSCs in Adipose Tissue Engineering. Int. J. Mol. Sci. 2012, 13, 15881-15900.

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