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Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE)
Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo, Spain
Present address: Institute of Biomedicine, University of Gothenburg, 41390 Gothenburg, Sweden
* Author to whom correspondence should be addressed.
Received: 1 August 2012; in revised form: 20 October 2012 / Accepted: 1 November 2012 / Published: 7 November 2012
Abstract: Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGFβIp), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.
Keywords: Caco-2; secretome; differentiation; DIGE; colorectal cancer; biomarkers
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García-Lorenzo, A.; Rodríguez-Piñeiro, A.M.; Rodríguez-Berrocal, F.J.; Cadena, M.P.; Martínez-Zorzano, V.S. Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE). Int. J. Mol. Sci. 2012, 13, 14401-14420.
García-Lorenzo A, Rodríguez-Piñeiro AM, Rodríguez-Berrocal FJ, Cadena MP, Martínez-Zorzano VS. Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE). International Journal of Molecular Sciences. 2012; 13(11):14401-14420.
García-Lorenzo, Andrés; Rodríguez-Piñeiro, Ana M.; Rodríguez-Berrocal, Francisco J.; Cadena, María P.; Martínez-Zorzano, Vicenta S. 2012. "Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE)." Int. J. Mol. Sci. 13, no. 11: 14401-14420.