Next Article in Journal
Endpoint Visual Detection of Three Genetically Modified Rice Events by Loop-Mediated Isothermal Amplification
Previous Article in Journal
Monitoring Biosensor Activity in Living Cells with Fluorescence Lifetime Imaging Microscopy
Int. J. Mol. Sci. 2012, 13(11), 14401-14420; doi:10.3390/ijms131114401

Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE)

Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo, Spain Present address: Institute of Biomedicine, University of Gothenburg, 41390 Gothenburg, Sweden
* Author to whom correspondence should be addressed.
Received: 1 August 2012 / Revised: 20 October 2012 / Accepted: 1 November 2012 / Published: 7 November 2012
(This article belongs to the Section Molecular Diagnostics)
View Full-Text   |   Download PDF [584 KB, uploaded 19 June 2014]   |   Browse Figures


Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGFβIp), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.
Keywords: Caco-2; secretome; differentiation; DIGE; colorectal cancer; biomarkers Caco-2; secretome; differentiation; DIGE; colorectal cancer; biomarkers
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary material

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

García-Lorenzo, A.; Rodríguez-Piñeiro, A.M.; Rodríguez-Berrocal, F.J.; Cadena, M.P.; Martínez-Zorzano, V.S. Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE). Int. J. Mol. Sci. 2012, 13, 14401-14420.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert