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Int. J. Mol. Sci. 2012, 13(11), 14401-14420; doi:10.3390/ijms131114401
Article

Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE)

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Received: 1 August 2012; in revised form: 20 October 2012 / Accepted: 1 November 2012 / Published: 7 November 2012
(This article belongs to the Section Molecular Pathology)
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Abstract: Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGFβIp), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.
Keywords: Caco-2; secretome; differentiation; DIGE; colorectal cancer; biomarkers Caco-2; secretome; differentiation; DIGE; colorectal cancer; biomarkers
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

García-Lorenzo, A.; Rodríguez-Piñeiro, A.M.; Rodríguez-Berrocal, F.J.; Cadena, M.P.; Martínez-Zorzano, V.S. Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE). Int. J. Mol. Sci. 2012, 13, 14401-14420.

AMA Style

García-Lorenzo A, Rodríguez-Piñeiro AM, Rodríguez-Berrocal FJ, Cadena MP, Martínez-Zorzano VS. Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE). International Journal of Molecular Sciences. 2012; 13(11):14401-14420.

Chicago/Turabian Style

García-Lorenzo, Andrés; Rodríguez-Piñeiro, Ana M.; Rodríguez-Berrocal, Francisco J.; Cadena, María P.; Martínez-Zorzano, Vicenta S. 2012. "Changes on the Caco-2 Secretome through Differentiation Analyzed by 2-D Differential In-Gel Electrophoresis (DIGE)." Int. J. Mol. Sci. 13, no. 11: 14401-14420.



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