Next Article in Journal
Effects of White Rice, Brown Rice and Germinated Brown Rice on Antioxidant Status of Type 2 Diabetic Rats
Next Article in Special Issue
Quantitative Structure-Activity Relationships Predicting the Antioxidant Potency of 17β-Estradiol-Related Polycyclic Phenols to Inhibit Lipid Peroxidation
Previous Article in Journal
Hyaluronan Synthase and Hyaluronidase Expression in Serous Ovarian Carcinoma is Related to Anatomic Site and Chemotherapy Exposure
Previous Article in Special Issue
Peripheral Nerve Injuries and Transplantation of Olfactory Ensheathing Cells for Axonal Regeneration and Remyelination: Fact or Fiction?
Int. J. Mol. Sci. 2012, 13(10), 12939-12951; doi:10.3390/ijms131012939
Article

Erythropoietin Modulates Autophagy Signaling in the Developing Rat Brain in an In Vivo Model of Oxygen-Toxicity

1,* , 2
, 2
, 3
, 4
, 5
, 1
 and 2
Received: 19 July 2012; in revised form: 28 August 2012 / Accepted: 21 September 2012 / Published: 10 October 2012
(This article belongs to the Special Issue Neuroprotective Strategies 2012)
View Full-Text   |   Download PDF [836 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Autophagy is a self-degradative process that involves turnover and recycling of cytoplasmic components in healthy and diseased tissue. Autophagy has been shown to be protective at the early stages of programmed cell death but it can also promote apoptosis under certain conditions. Earlier we demonstrated that oxygen contributes to the pathogenesis of neonatal brain damage, which can be ameliorated by intervention with recombinant human erythropoietin (rhEpo). Extrinsic- and intrinsic apoptotic pathways are involved in oxygen induced neurotoxicity but the role of autophagy in this model is unclear. We analyzed the expression of autophagy activity markers in the immature rodent brain after exposure to elevated oxygen concentrations. We observed a hyperoxia-exposure dependent regulation of autophagy-related gene (Atg) proteins Atg3, 5, 12, Beclin-1, microtubule-associated protein 1 light chain 3 (LC3), LC3A-II, and LC3B-II which are all key autophagy activity proteins. Interestingly, a single injection with rhEpo at the onset of hyperoxia counteracted these oxygen-mediated effects. Our results indicate that rhEpo generates its protective effect by modifying the key autophagy activity proteins.
Keywords: autophagy; developing brain; oxidative stress; hyperoxia; erythropoietin autophagy; developing brain; oxidative stress; hyperoxia; erythropoietin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Bendix, I.; Schulze, C.; Haefen, C.; Gellhaus, A.; Endesfelder, S.; Heumann, R.; Felderhoff-Mueser, U.; Sifringer, M. Erythropoietin Modulates Autophagy Signaling in the Developing Rat Brain in an In Vivo Model of Oxygen-Toxicity. Int. J. Mol. Sci. 2012, 13, 12939-12951.

AMA Style

Bendix I, Schulze C, Haefen C, Gellhaus A, Endesfelder S, Heumann R, Felderhoff-Mueser U, Sifringer M. Erythropoietin Modulates Autophagy Signaling in the Developing Rat Brain in an In Vivo Model of Oxygen-Toxicity. International Journal of Molecular Sciences. 2012; 13(10):12939-12951.

Chicago/Turabian Style

Bendix, Ivo; Schulze, Corina; Haefen, Clarissa von; Gellhaus, Alexandra; Endesfelder, Stefanie; Heumann, Rolf; Felderhoff-Mueser, Ursula; Sifringer, Marco. 2012. "Erythropoietin Modulates Autophagy Signaling in the Developing Rat Brain in an In Vivo Model of Oxygen-Toxicity." Int. J. Mol. Sci. 13, no. 10: 12939-12951.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert