Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2012, 13(10), 12349-12366; doi:10.3390/ijms131012349
Article

Mercuric Compounds Induce Pancreatic Islets Dysfunction and Apoptosis in Vivo

1,†
, 2,†
, 3,†
, 4,†
, 5
, 6
, 7
, 8
, 8
, 9
 and 9,*
Received: 2 July 2012; in revised form: 2 September 2012 / Accepted: 17 September 2012 / Published: 26 September 2012
View Full-Text   |   Download PDF [851 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Mercury is a toxic heavy metal that is an environmental and industrial pollutant throughout the world. Mercury exposure leads to many physiopathological injuries in mammals. However, the precise toxicological effects of mercury on pancreatic islets in vivo are still unclear. Here, we investigated whether mercuric compounds can induce dysfunction and damage in the pancreatic islets of mice, as well as the possible mechanisms involved in this process. Mice were treated with methyl mercuric chloride (MeHgCl, 2 mg/kg) and mercuric chloride (HgCl2, 5 mg/kg) for more than 2 consecutive weeks. Our results showed that the blood glucose levels increased and plasma insulin secretions decreased in the mice as a consequence of their exposure. A significant number of TUNEL-positive cells were revealed in the islets of mice that were treated with mercury for 2 consecutive weeks, which was accompanied by changes in the expression of the mRNA of anti-apoptotic (Bcl-2, Mcl-1, and Mdm-2) and apoptotic (p53, caspase-3, and caspase-7) genes. Moreover, plasma malondialdehyde (MDA) levels increased significantly in the mice after treatment with mercuric compounds for 2 consecutive weeks, and the generation of reactive oxygen species (ROS) in the pancreatic islets also markedly increased. In addition, the mRNA expression of genes related to antioxidation, including Nrf2, GPx, and NQO1, were also significantly reduced in these islets. These results indicate that oxidative stress injuries that are induced by mercuric compounds can cause pancreatic islets dysfunction and apoptosis in vivo.
Keywords: mercuric compounds; pancreatic islets; oxidative stress; apoptosis mercuric compounds; pancreatic islets; oxidative stress; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Chen, K.-L.; Liu, S.-H.; Su, C.-C.; Yen, C.-C.; Yang, C.-Y.; Lee, K.-I.; Tang, F.-C.; Chen, Y.-W.; Lu, T.-H.; Su, Y.-C.; Huang, C.-F. Mercuric Compounds Induce Pancreatic Islets Dysfunction and Apoptosis in Vivo. Int. J. Mol. Sci. 2012, 13, 12349-12366.

AMA Style

Chen K-L, Liu S-H, Su C-C, Yen C-C, Yang C-Y, Lee K-I, Tang F-C, Chen Y-W, Lu T-H, Su Y-C, Huang C-F. Mercuric Compounds Induce Pancreatic Islets Dysfunction and Apoptosis in Vivo. International Journal of Molecular Sciences. 2012; 13(10):12349-12366.

Chicago/Turabian Style

Chen, Kuo-Liang; Liu, Shing-Hwa; Su, Chin-Chuan; Yen, Cheng-Chieh; Yang, Ching-Yao; Lee, Kuan-I; Tang, Feng-Cheng; Chen, Ya-Wen; Lu, Tien-Hui; Su, Yi-Chang; Huang, Chun-Fa. 2012. "Mercuric Compounds Induce Pancreatic Islets Dysfunction and Apoptosis in Vivo." Int. J. Mol. Sci. 13, no. 10: 12349-12366.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert