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Int. J. Mol. Sci. 2011, 12(9), 5828-5843; doi:10.3390/ijms12095828

Efficient 1H-NMR Quantitation and Investigation of N-Acetyl-D-glucosamine (GlcNAc) and N,N'-Diacetylchitobiose (GlcNAc)2 from Chitin

1
Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan
2
Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan
3
Department of Pharmacy, China Medical University, Taichung 401, Taiwan
*
Author to whom correspondence should be addressed.
Received: 20 June 2011 / Revised: 22 August 2011 / Accepted: 26 August 2011 / Published: 9 September 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

A quantitative determination method of N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose (GlcNAc)2 is proposed using a proton nuclear magnetic resonance experiment. N-acetyl groups of GlcNAc and (GlcNAc)2 are chosen as target signals, and the deconvolution technique is used to determine the concentration of the corresponding compound. Compared to the HPLC method, 1H-NMR spectroscopy is simple and fast. The method can be used for the analysis of chitin hydrolyzed products with real-time analysis, and for quantifying the content of products using internal standards without calibration curves. This method can be used to quickly evaluate chitinase activity. The temperature dependence of 1H-NMR spectra (VT-NMR) is studied to monitor the chemical shift variation of acetyl peak. The acetyl groups of products are involved in intramolecular H-bonding with the OH group on anomeric sites. The rotation of the acetyl group is closely related to the intramolecular hydrogen bonding pattern, as suggested by the theoretical data (molecular modeling).
Keywords: nuclear magnetic resonance; hydrolysis; kinetics; chitin; enzyme nuclear magnetic resonance; hydrolysis; kinetics; chitin; enzyme
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Liu, F.-C.; Su, C.-R.; Wu, T.-Y.; Su, S.-G.; Yang, H.-L.; Lin, J.-Y.; Wu, T.-S. Efficient 1H-NMR Quantitation and Investigation of N-Acetyl-D-glucosamine (GlcNAc) and N,N'-Diacetylchitobiose (GlcNAc)2 from Chitin. Int. J. Mol. Sci. 2011, 12, 5828-5843.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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