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Int. J. Mol. Sci. 2011, 12(7), 4522-4535; doi:10.3390/ijms12074522
Article

Antiviral Activity and Mechanism of Action of Novel Thiourea Containing Chiral Phosphonate on Tobacco Mosaic Virus

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Received: 30 May 2011 / Revised: 18 June 2011 / Accepted: 4 July 2011 / Published: 13 July 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Using half-leaf method O,O’-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3-phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K326 (Nicotiana tabacum K326) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.
Keywords: chiral thiourea compounds; tobacco mosaic virus; antiviral activity; mechanism of action chiral thiourea compounds; tobacco mosaic virus; antiviral activity; mechanism of action
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fan, H.; Song, B.; Bhadury, P.S.; Jin, L.; Hu, D.; Yang, S. Antiviral Activity and Mechanism of Action of Novel Thiourea Containing Chiral Phosphonate on Tobacco Mosaic Virus. Int. J. Mol. Sci. 2011, 12, 4522-4535.

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