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To Remove or Not to Remove? The Challenge of Extracting the Template to Make the Cavities Available in Molecularly Imprinted Polymers (MIPs)
Department Química Analítica, Nutrición y Bromatología, Facultad de Química, Universidad de Santiago de Compostela, Avda. de las Ciencias, s/n, 15782-Santiago de Compostela, Spain
Department Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782-Santiago de Compostela, Spain
* Author to whom correspondence should be addressed.
Received: 2 June 2011; in revised form: 23 June 2011 / Accepted: 29 June 2011 / Published: 5 July 2011
Abstract: Template removal is a critical step in the preparation of most molecularly imprinted polymers (MIPs). The polymer network itself and the affinity of the imprinted cavities for the template make its removal hard. If there are remaining template molecules in the MIPs, less cavities will be available for rebinding, which decreases efficiency. Furthermore, if template bleeding occurs during analytical applications, errors will arise. Despite the relevance to the MIPs performance, template removal has received scarce attention and is currently the least cost-effective step of the MIP development. Attempts to reach complete template removal may involve the use of too drastic conditions in conventional extraction techniques, resulting in the damage or the collapse of the imprinted cavities. Advances in the extraction techniques in the last decade may provide optimized tools. The aim of this review is to analyze the available data on the efficiency of diverse extraction techniques for template removal, paying attention not only to the removal yield but also to MIPs performance. Such an analysis is expected to be useful for opening a way to rational approaches for template removal (minimizing the costs of solvents and time) instead of the current trial-and-error methods.
Keywords: molecularly imprinted polymer (MIP); template removal; solvent extraction; physically-assisted extraction; ultrasound-assisted extraction; microwave-assisted extraction; pressurized-liquid extraction; subcritical water extraction; supercritical fluids extraction
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Cite This Article
MDPI and ACS Style
Lorenzo, R.A.; Carro, A.M.; Alvarez-Lorenzo, C.; Concheiro, A. To Remove or Not to Remove? The Challenge of Extracting the Template to Make the Cavities Available in Molecularly Imprinted Polymers (MIPs). Int. J. Mol. Sci. 2011, 12, 4327-4347.
Lorenzo RA, Carro AM, Alvarez-Lorenzo C, Concheiro A. To Remove or Not to Remove? The Challenge of Extracting the Template to Make the Cavities Available in Molecularly Imprinted Polymers (MIPs). International Journal of Molecular Sciences. 2011; 12(7):4327-4347.
Lorenzo, Rosa A.; Carro, Antonia M.; Alvarez-Lorenzo, Carmen; Concheiro, Angel. 2011. "To Remove or Not to Remove? The Challenge of Extracting the Template to Make the Cavities Available in Molecularly Imprinted Polymers (MIPs)." Int. J. Mol. Sci. 12, no. 7: 4327-4347.