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Int. J. Mol. Sci. 2011, 12(6), 3998-4008; doi:10.3390/ijms12063998

The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase

1
Department of Life Science, College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Tokyo, Japan
2
Department of Applied Biological Science, Faculty of Agriculture, Shizuoka University, 836 Oya, Shizuoka, Japan
*
Author to whom correspondence should be addressed.
Received: 25 April 2011 / Revised: 31 May 2011 / Accepted: 8 June 2011 / Published: 14 June 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

In the present work, we investigated the effect of non-steroidal anti-inflammatory drugs (NSAIDs) on the monophenolase and diphenolase activity of mushroom tyrosinase. The results showed that diflunisal and indomethacin inhibited both monophenolase and diphenolase activity. For monophenolase activity, the lag time was extended in the presence of diflunisal. In the presence of indomethacin, the lag time did not change. IC50 values of monophenolase activity were estimated to be 0.112 mM (diflunisal) and 1.78 mM (indomethacin). Kinetic studies of monophenolase activity revealed that both diflunisal and indomethacin were non-competitive inhibitors. For diphenolase activity, IC50 values were estimated to be 0.197 mM (diflunisal) and 0.509 mM (indomethacin). Diflunisal and indomethacin were also found to be non-competitive diphenolase inhibitors. View Full-Text
Keywords: tyrosinase; monophenolase; diphenolase; NSAIDs (non-steroidal anti-inflammatory drugs); diflunisal; indomethacin tyrosinase; monophenolase; diphenolase; NSAIDs (non-steroidal anti-inflammatory drugs); diflunisal; indomethacin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sato, K.; Toriyama, M. The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase. Int. J. Mol. Sci. 2011, 12, 3998-4008.

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