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Int. J. Mol. Sci. 2011, 12(6), 3998-4008; doi:10.3390/ijms12063998
Article

The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase

1,*  and 2
Received: 25 April 2011; in revised form: 31 May 2011 / Accepted: 8 June 2011 / Published: 14 June 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: In the present work, we investigated the effect of non-steroidal anti-inflammatory drugs (NSAIDs) on the monophenolase and diphenolase activity of mushroom tyrosinase. The results showed that diflunisal and indomethacin inhibited both monophenolase and diphenolase activity. For monophenolase activity, the lag time was extended in the presence of diflunisal. In the presence of indomethacin, the lag time did not change. IC50 values of monophenolase activity were estimated to be 0.112 mM (diflunisal) and 1.78 mM (indomethacin). Kinetic studies of monophenolase activity revealed that both diflunisal and indomethacin were non-competitive inhibitors. For diphenolase activity, IC50 values were estimated to be 0.197 mM (diflunisal) and 0.509 mM (indomethacin). Diflunisal and indomethacin were also found to be non-competitive diphenolase inhibitors.
Keywords: tyrosinase; monophenolase; diphenolase; NSAIDs (non-steroidal anti-inflammatory drugs); diflunisal; indomethacin tyrosinase; monophenolase; diphenolase; NSAIDs (non-steroidal anti-inflammatory drugs); diflunisal; indomethacin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sato, K.; Toriyama, M. The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase. Int. J. Mol. Sci. 2011, 12, 3998-4008.

AMA Style

Sato K, Toriyama M. The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase. International Journal of Molecular Sciences. 2011; 12(6):3998-4008.

Chicago/Turabian Style

Sato, Kazuomi; Toriyama, Masaru. 2011. "The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase." Int. J. Mol. Sci. 12, no. 6: 3998-4008.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert