Next Article in Journal
Molecular Characterization and Expression Pattern of Tripartite Motif Protein 39 in Gallus gallus with a Complete PRY/SPRY Domain
Previous Article in Journal
Differences in Soil Properties and Bacterial Communities between the Rhizosphere and Bulk Soil and among Different Production Areas of the Medicinal Plant Fritillaria thunbergii
Int. J. Mol. Sci. 2011, 12(6), 3786-3796; doi:10.3390/ijms12063786
Article

Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002

1
, 1,*  and 2
Received: 28 February 2011; in revised form: 12 May 2011 / Accepted: 25 May 2011 / Published: 9 June 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [422 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Rutin was subjected to intermolecular transglycosylation assisted with microwave irradiation using cyclodextrin glucanotransferase (CGTase) produced from Bacillus sp. SK13.002. Compared with the conventional enzymatic method for rutin transglycosylation (without microwave irradiation), microwave-assisted reaction (MAR) was much faster and thus more efficient. While the conventional reaction took dozens of hours to reach the highest conversion rate of rutin and yield of transglycosylated rutin, MAR of rutin transglycosylation completed within only 6 min providing almost the same conversion rate of rutin and yield of products consisting of mono-, di-, tri-, tetra-, penta-glucosylated rutins. The optimum transglycosylation conditions for microwave irradiation were 40 °C and 60 W with the reaction system consisting mainly of the mixture of 0.3 g rutin (0.49 mmol) pre-dissolved in 15 mL methanol, 1.8 g maltodextrin in 15 mL of 0.2 M sodium acetate buffer (pH 5.5) and CGTase (900 U). Results from this study indicated that MAR could be a potentially useful and economical technique for a faster and more efficient transglycosylation of rutin.
Keywords: rutin; cyclodextrin glucanotransferase; microwave-assisted reaction; transglycosylation rutin; cyclodextrin glucanotransferase; microwave-assisted reaction; transglycosylation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sun, T.; Jiang, B.; Pan, B. Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002. Int. J. Mol. Sci. 2011, 12, 3786-3796.

AMA Style

Sun T, Jiang B, Pan B. Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002. International Journal of Molecular Sciences. 2011; 12(6):3786-3796.

Chicago/Turabian Style

Sun, Tao; Jiang, Bo; Pan, Beilei. 2011. "Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002." Int. J. Mol. Sci. 12, no. 6: 3786-3796.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert