Int. J. Mol. Sci. 2011, 12(6), 3786-3796; doi:10.3390/ijms12063786
Article

Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002

1email, 1,* email and 2email
1 State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China 2 Chinese Institute of Food Science and Technology, Beijing 100006, China
* Author to whom correspondence should be addressed.
Received: 28 February 2011; in revised form: 12 May 2011 / Accepted: 25 May 2011 / Published: 9 June 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Rutin was subjected to intermolecular transglycosylation assisted with microwave irradiation using cyclodextrin glucanotransferase (CGTase) produced from Bacillus sp. SK13.002. Compared with the conventional enzymatic method for rutin transglycosylation (without microwave irradiation), microwave-assisted reaction (MAR) was much faster and thus more efficient. While the conventional reaction took dozens of hours to reach the highest conversion rate of rutin and yield of transglycosylated rutin, MAR of rutin transglycosylation completed within only 6 min providing almost the same conversion rate of rutin and yield of products consisting of mono-, di-, tri-, tetra-, penta-glucosylated rutins. The optimum transglycosylation conditions for microwave irradiation were 40 °C and 60 W with the reaction system consisting mainly of the mixture of 0.3 g rutin (0.49 mmol) pre-dissolved in 15 mL methanol, 1.8 g maltodextrin in 15 mL of 0.2 M sodium acetate buffer (pH 5.5) and CGTase (900 U). Results from this study indicated that MAR could be a potentially useful and economical technique for a faster and more efficient transglycosylation of rutin.
Keywords: rutin; cyclodextrin glucanotransferase; microwave-assisted reaction; transglycosylation

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MDPI and ACS Style

Sun, T.; Jiang, B.; Pan, B. Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002. Int. J. Mol. Sci. 2011, 12, 3786-3796.

AMA Style

Sun T, Jiang B, Pan B. Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002. International Journal of Molecular Sciences. 2011; 12(6):3786-3796.

Chicago/Turabian Style

Sun, Tao; Jiang, Bo; Pan, Beilei. 2011. "Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002." Int. J. Mol. Sci. 12, no. 6: 3786-3796.

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