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Int. J. Mol. Sci. 2011, 12(6), 3489-3499; doi:10.3390/ijms12063489
Article

Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer

1,†
, 2,†
, 3
, 3
, 4,*  and 3,*
Received: 30 March 2011; in revised form: 17 May 2011 / Accepted: 24 May 2011 / Published: 1 June 2011
(This article belongs to the Special Issue Biomarkers 2011)
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Abstract: The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identified, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.
Keywords: non-small lung cancer; cisplatin; resistance; DJ-1; proteomics non-small lung cancer; cisplatin; resistance; DJ-1; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zeng, H.-Z.; Qu, Y.-Q.; Zhang, W.-J.; Xiu, B.; Deng, A.-M.; Liang, A.-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2011, 12, 3489-3499.

AMA Style

Zeng H-Z, Qu Y-Q, Zhang W-J, Xiu B, Deng A-M, Liang A-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. International Journal of Molecular Sciences. 2011; 12(6):3489-3499.

Chicago/Turabian Style

Zeng, Hua-Zong; Qu, Yi-Qing; Zhang, Wen-Jun; Xiu, Bing; Deng, An-Mei; Liang, Ai-Bin. 2011. "Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer." Int. J. Mol. Sci. 12, no. 6: 3489-3499.


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