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Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer
School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
Department of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan 250012, China
Central Laboratory, Tongji Hospital of Tongji University, Tongji University, Shanghai 200065, China
Laboratory Diagnostics, Shanghai Changhai Hospital, Shanghai 200003, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 30 March 2011; in revised form: 17 May 2011 / Accepted: 24 May 2011 / Published: 1 June 2011
Abstract: The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identiﬁed, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.
Keywords: non-small lung cancer; cisplatin; resistance; DJ-1; proteomics
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MDPI and ACS Style
Zeng, H.-Z.; Qu, Y.-Q.; Zhang, W.-J.; Xiu, B.; Deng, A.-M.; Liang, A.-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2011, 12, 3489-3499.
Zeng H-Z, Qu Y-Q, Zhang W-J, Xiu B, Deng A-M, Liang A-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. International Journal of Molecular Sciences. 2011; 12(6):3489-3499.
Zeng, Hua-Zong; Qu, Yi-Qing; Zhang, Wen-Jun; Xiu, Bing; Deng, An-Mei; Liang, Ai-Bin. 2011. "Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer." Int. J. Mol. Sci. 12, no. 6: 3489-3499.