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Int. J. Mol. Sci. 2011, 12(6), 3489-3499; doi:10.3390/ijms12063489

Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer

4,*  and 3,*
1 School of Life Sciences and Technology, Tongji University, Shanghai 200092, China 2 Department of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan 250012, China 3 Central Laboratory, Tongji Hospital of Tongji University, Tongji University, Shanghai 200065, China 4 Laboratory Diagnostics, Shanghai Changhai Hospital, Shanghai 200003, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 30 March 2011 / Revised: 17 May 2011 / Accepted: 24 May 2011 / Published: 1 June 2011
(This article belongs to the Special Issue Biomarkers 2011)
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The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identified, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.
Keywords: non-small lung cancer; cisplatin; resistance; DJ-1; proteomics non-small lung cancer; cisplatin; resistance; DJ-1; proteomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zeng, H.-Z.; Qu, Y.-Q.; Zhang, W.-J.; Xiu, B.; Deng, A.-M.; Liang, A.-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2011, 12, 3489-3499.

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