Next Article in Journal
Optimal Designs of Staggered Dean Vortex Micromixers
Next Article in Special Issue
Putative Biomarkers and Targets of Estrogen Receptor Negative Human Breast Cancer
Previous Article in Journal
Resistance to Elsinoë Ampelina and Expression of Related Resistant Genes in Vitis Rotundifolia Michx. Grapes
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2011, 12(6), 3489-3499; doi:10.3390/ijms12063489

Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer

School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
Department of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan 250012, China
Central Laboratory, Tongji Hospital of Tongji University, Tongji University, Shanghai 200065, China
Laboratory Diagnostics, Shanghai Changhai Hospital, Shanghai 200003, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 30 March 2011 / Revised: 17 May 2011 / Accepted: 24 May 2011 / Published: 1 June 2011
(This article belongs to the Special Issue Biomarkers 2011)
View Full-Text   |   Download PDF [497 KB, 19 June 2014; original version 19 June 2014]   |  


The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identified, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.
Keywords: non-small lung cancer; cisplatin; resistance; DJ-1; proteomics non-small lung cancer; cisplatin; resistance; DJ-1; proteomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zeng, H.-Z.; Qu, Y.-Q.; Zhang, W.-J.; Xiu, B.; Deng, A.-M.; Liang, A.-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2011, 12, 3489-3499.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top