Abstract: The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identiﬁed, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.
Keywords: non-small lung cancer; cisplatin; resistance; DJ-1; proteomics
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Zeng, H.-Z.; Qu, Y.-Q.; Zhang, W.-J.; Xiu, B.; Deng, A.-M.; Liang, A.-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2011, 12, 3489-3499.
Zeng H-Z, Qu Y-Q, Zhang W-J, Xiu B, Deng A-M, Liang A-B. Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer. International Journal of Molecular Sciences. 2011; 12(6):3489-3499.
Zeng, Hua-Zong; Qu, Yi-Qing; Zhang, Wen-Jun; Xiu, Bing; Deng, An-Mei; Liang, Ai-Bin. 2011. "Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer." Int. J. Mol. Sci. 12, no. 6: 3489-3499.