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Production and Radioprotective Effects of Pyrroloquinoline Quinone
Laboratory of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China
Central Laboratory, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China
Department of Pharmacology, Beijing Institute of Radiation Medicine, Beijing 100850, China
School of Life Science and Biochemical Pharmaceutical, Shenyang Pharmaceutical University, Shenyang 110016, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 28 October 2011; in revised form: 22 November 2011 / Accepted: 24 November 2011 / Published: 5 December 2011
Abstract: Pyrroloquinoline quinone (PQQ) was produced by fermentation of the Methylovorus sp. MP688 strain and purified by ion-exchange chromatography, crystallization and recrystallization. The yield of PQQ reached approximately 125 mg/L and highly pure PQQ was obtained. To determine the optimum dose of PQQ for radioprotection, three doses (2 mg/kg, 4 mg/kg, 8 mg/kg) of PQQ were orally administrated to the experimental animals subjected to a lethal dose of 8.0 Gy in survival test. Survival of mice in the irradiation + PQQ (4 mg/kg) group was found to be significantly higher in comparison with the irradiation and irradiation + nilestriol (10 mg/kg) groups. The numbers of hematocytes and bone marrow cells were measured for 21 days after sublethal 4 Gy gamma-ray irradiation with per os of 4 mg/kg of PQQ. The recovery of white blood cells, reticulocytes and bone marrow cells in the irradiation + PQQ group was faster than that in the irradiation group. Furthermore, the recovery of bone marrow cell in the irradiation + PQQ group was superior to that in irradiation + nilestriol group. Our results clearly indicate favourable effects on survival under higher lethal radiation doses and the ability of pyrroloquinoline quinine to enhance haemopoietic recovery after sublethal radiation exposure.
Keywords: methylotroph; pyrroloquinoline quinine; radioprotective; hematopoiesis; survival
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Xiong, X.-H.; Zhao, Y.; Ge, X.; Yuan, S.-J.; Wang, J.-H.; Zhi, J.-J.; Yang, Y.-X.; Du, B.-H.; Guo, W.-J.; Wang, S.-S.; Yang, D.-X.; Zhang, W.-C. Production and Radioprotective Effects of Pyrroloquinoline Quinone. Int. J. Mol. Sci. 2011, 12, 8913-8923.
Xiong X-H, Zhao Y, Ge X, Yuan S-J, Wang J-H, Zhi J-J, Yang Y-X, Du B-H, Guo W-J, Wang S-S, Yang D-X, Zhang W-C. Production and Radioprotective Effects of Pyrroloquinoline Quinone. International Journal of Molecular Sciences. 2011; 12(12):8913-8923.
Xiong, Xiang-Hua; Zhao, Yan; Ge, Xin; Yuan, Shou-Jun; Wang, Jian-Hua; Zhi, Jing-Juan; Yang, Yan-Xin; Du, Bao-Hua; Guo, Wan-Jun; Wang, Shan-Shan; Yang, De-Xuan; Zhang, Wei-Cai. 2011. "Production and Radioprotective Effects of Pyrroloquinoline Quinone." Int. J. Mol. Sci. 12, no. 12: 8913-8923.