Abstract: Pyrroloquinoline quinone (PQQ) was produced by fermentation of the Methylovorus sp. MP688 strain and purified by ion-exchange chromatography, crystallization and recrystallization. The yield of PQQ reached approximately 125 mg/L and highly pure PQQ was obtained. To determine the optimum dose of PQQ for radioprotection, three doses (2 mg/kg, 4 mg/kg, 8 mg/kg) of PQQ were orally administrated to the experimental animals subjected to a lethal dose of 8.0 Gy in survival test. Survival of mice in the irradiation + PQQ (4 mg/kg) group was found to be significantly higher in comparison with the irradiation and irradiation + nilestriol (10 mg/kg) groups. The numbers of hematocytes and bone marrow cells were measured for 21 days after sublethal 4 Gy gamma-ray irradiation with per os of 4 mg/kg of PQQ. The recovery of white blood cells, reticulocytes and bone marrow cells in the irradiation + PQQ group was faster than that in the irradiation group. Furthermore, the recovery of bone marrow cell in the irradiation + PQQ group was superior to that in irradiation + nilestriol group. Our results clearly indicate favourable effects on survival under higher lethal radiation doses and the ability of pyrroloquinoline quinine to enhance haemopoietic recovery after sublethal radiation exposure.
Keywords: methylotroph; pyrroloquinoline quinine; radioprotective; hematopoiesis; survival
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Xiong, X.-H.; Zhao, Y.; Ge, X.; Yuan, S.-J.; Wang, J.-H.; Zhi, J.-J.; Yang, Y.-X.; Du, B.-H.; Guo, W.-J.; Wang, S.-S.; Yang, D.-X.; Zhang, W.-C. Production and Radioprotective Effects of Pyrroloquinoline Quinone. Int. J. Mol. Sci. 2011, 12, 8913-8923.
Xiong X-H, Zhao Y, Ge X, Yuan S-J, Wang J-H, Zhi J-J, Yang Y-X, Du B-H, Guo W-J, Wang S-S, Yang D-X, Zhang W-C. Production and Radioprotective Effects of Pyrroloquinoline Quinone. International Journal of Molecular Sciences. 2011; 12(12):8913-8923.
Xiong, Xiang-Hua; Zhao, Yan; Ge, Xin; Yuan, Shou-Jun; Wang, Jian-Hua; Zhi, Jing-Juan; Yang, Yan-Xin; Du, Bao-Hua; Guo, Wan-Jun; Wang, Shan-Shan; Yang, De-Xuan; Zhang, Wei-Cai. 2011. "Production and Radioprotective Effects of Pyrroloquinoline Quinone." Int. J. Mol. Sci. 12, no. 12: 8913-8923.