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• Xiong, X
• Zhao, Y
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• Yang, D
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• Yang, D
• Zhang, W
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• Zhao, Y
• Ge, X
• Yuan, S
• Wang, J
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Int. J. Mol. Sci. 2011, 12(12), 8913-8923; doi:10.3390/ijms12128913

Article

Production and Radioprotective Effects of Pyrroloquinoline Quinone

Xiang-Hua Xiong ^1,† , Yan Zhao ^2,† , Xin Ge ^1,† , Shou-Jun Yuan ³ , Jian-Hua Wang ¹ , Jing-Juan Zhi ⁴ , Yan-Xin Yang ⁴ , Bao-Hua Du ⁴ , Wan-Jun Guo ³ , Shan-Shan Wang ³ , De-Xuan Yang ³  and Wei-Cai Zhang ^1,*

¹ Laboratory of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing 100071, China ² Central Laboratory, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China ³ Department of Pharmacology, Beijing Institute of Radiation Medicine, Beijing 100850, China ⁴ School of Life Science and Biochemical Pharmaceutical, Shenyang Pharmaceutical University, Shenyang 110016, China ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 28 October 2011; in revised form: 22 November 2011 / Accepted: 24 November 2011 / Published: 5 December 2011

(This article belongs to the Section Bioactives and Nutraceuticals)

Download PDF Full-Text [144 KB, uploaded 5 December 2011 09:30 CET]

Abstract: Pyrroloquinoline quinone (PQQ) was produced by fermentation of the Methylovorus sp. MP688 strain and purified by ion-exchange chromatography, crystallization and recrystallization. The yield of PQQ reached approximately 125 mg/L and highly pure PQQ was obtained. To determine the optimum dose of PQQ for radioprotection, three doses (2 mg/kg, 4 mg/kg, 8 mg/kg) of PQQ were orally administrated to the experimental animals subjected to a lethal dose of 8.0 Gy in survival test. Survival of mice in the irradiation + PQQ (4 mg/kg) group was found to be significantly higher in comparison with the irradiation and irradiation + nilestriol (10 mg/kg) groups. The numbers of hematocytes and bone marrow cells were measured for 21 days after sublethal 4 Gy gamma-ray irradiation with per os of 4 mg/kg of PQQ. The recovery of white blood cells, reticulocytes and bone marrow cells in the irradiation + PQQ group was faster than that in the irradiation group. Furthermore, the recovery of bone marrow cell in the irradiation + PQQ group was superior to that in irradiation + nilestriol group. Our results clearly indicate favourable effects on survival under higher lethal radiation doses and the ability of pyrroloquinoline quinine to enhance haemopoietic recovery after sublethal radiation exposure.

Keywords: methylotroph; pyrroloquinoline quinine; radioprotective; hematopoiesis; survival

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