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Knockdown of Ephrin-A5 Expression by 40% Does not Affect Motor Axon Growth or Migration into the Chick Hindlimb
Biology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA
The Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, 5211 Dental, 1011 N. University Avenue, Ann Arbor, MI 48109, USA
* Author to whom correspondence should be addressed.
Received: 20 October 2011; in revised form: 9 November 2011 / Accepted: 11 November 2011 / Published: 29 November 2011
Abstract: Bidirectional signaling between Eph receptor tyrosine kinases and their cell-surface protein signals, the ephrins, comprises one mechanism for guiding motor axons to their proper targets. During projection of motor axons from the lateral motor column (LMC) motor neurons of the spinal cord to the hindlimb muscles in chick embryos, ephrin-A5 has been shown to be expressed in the LMC motor axons until they reach the base of the limb bud and initiate sorting into their presumptive dorsal and ventral nerve trunks, at which point expression is extinguished. We tested the hypothesis that this dynamic pattern of ephrin-A5 expression in LMC motor axons is important for the growth and guidance of the axons to, and into, the hindlimb by knocking down endogenous ephrin-A5 expression in the motor neurons and their axons. No perturbation of LMC motor axon projections was observed in response to this treatment, suggesting that ephrin-A5 is not needed for LMC motor axon growth or guidance.
Keywords: chick; motor neurons; in ovo electroporation
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Winning, R.S.; Krull, C.E. Knockdown of Ephrin-A5 Expression by 40% Does not Affect Motor Axon Growth or Migration into the Chick Hindlimb. Int. J. Mol. Sci. 2011, 12, 8362-8371.
Winning RS, Krull CE. Knockdown of Ephrin-A5 Expression by 40% Does not Affect Motor Axon Growth or Migration into the Chick Hindlimb. International Journal of Molecular Sciences. 2011; 12(12):8362-8371.
Winning, Robert S.; Krull, Catherine E. 2011. "Knockdown of Ephrin-A5 Expression by 40% Does not Affect Motor Axon Growth or Migration into the Chick Hindlimb." Int. J. Mol. Sci. 12, no. 12: 8362-8371.