Int. J. Mol. Sci. 2011, 12(11), 8052-8062; doi:10.3390/ijms12118052
Article

3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence

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Received: 21 October 2011; in revised form: 2 November 2011 / Accepted: 7 November 2011 / Published: 16 November 2011
(This article belongs to the Special Issue Applications of Circular Dichroism)
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Abstract: Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3′-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.
Keywords: Cy7 cyanine dye; left-handed Z-DNA; circular dichroism; DNA recognition; sensing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Choi, J.K.; D’Urso, A.; Trauernicht, M.; Shabbir-Hussain, M.; Holmes, A.E.; Balaz, M. 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence. Int. J. Mol. Sci. 2011, 12, 8052-8062.

AMA Style

Choi JK, D’Urso A, Trauernicht M, Shabbir-Hussain M, Holmes AE, Balaz M. 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence. International Journal of Molecular Sciences. 2011; 12(11):8052-8062.

Chicago/Turabian Style

Choi, Jung Kyu; D’Urso, Alessandro; Trauernicht, Mitch; Shabbir-Hussain, Murtaza; Holmes, Andrea E.; Balaz, Milan. 2011. "3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence." Int. J. Mol. Sci. 12, no. 11: 8052-8062.


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