Int. J. Mol. Sci. 2011, 12(11), 8052-8062; doi:10.3390/ijms12118052
Article

3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence

Jung Kyu Choi 1 email, Alessandro D’Urso 1,2 email, Mitch Trauernicht 2 email, Murtaza Shabbir-Hussain 1 email, Andrea E. Holmes 2 email and Milan Balaz 1,* email
1 Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA 2 Doane College, 1014 Boswell, Crete, NE 68333, USA
* Author to whom correspondence should be addressed.
Received: 21 October 2011; in revised form: 2 November 2011 / Accepted: 7 November 2011 / Published: 16 November 2011
(This article belongs to the Special Issue Applications of Circular Dichroism)
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Abstract: Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3′-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.
Keywords: Cy7 cyanine dye; left-handed Z-DNA; circular dichroism; DNA recognition; sensing

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MDPI and ACS Style

Choi, J.K.; D’Urso, A.; Trauernicht, M.; Shabbir-Hussain, M.; Holmes, A.E.; Balaz, M. 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence. Int. J. Mol. Sci. 2011, 12, 8052-8062.

AMA Style

Choi JK, D’Urso A, Trauernicht M, Shabbir-Hussain M, Holmes AE, Balaz M. 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence. International Journal of Molecular Sciences. 2011; 12(11):8052-8062.

Chicago/Turabian Style

Choi, Jung Kyu; D’Urso, Alessandro; Trauernicht, Mitch; Shabbir-Hussain, Murtaza; Holmes, Andrea E.; Balaz, Milan. 2011. "3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence." Int. J. Mol. Sci. 12, no. 11: 8052-8062.

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