Int. J. Mol. Sci. 2011, 12(11), 7950-7959; doi:10.3390/ijms12117950

MicroRNAs miR-27a and miR-143 Regulate Porcine Adipocyte Lipid Metabolism

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Received: 23 September 2011; in revised form: 7 November 2011 / Accepted: 8 November 2011 / Published: 15 November 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: MicroRNAs (miRNAs) are non-coding small RNAs that play roles in regulating gene expression. Some miRNAs have been classed as epigenetic regulators of metabolism and energy homeostasis. Previous reports indicated that the miRNAs miR-27a and miR-143 were involved in lipid metabolism in human and rodents. To determine the roles of porcine miR-27a and miR-143 in adipocyte lipid metabolism, porcine adipocytes were cultured and allowed to induce differentiation for 10 days. The lipid-filled adipocytes were then transfected with miRNA mimics and inhibitors. We measured how the indicators of adipogenesis and adipolysis in porcine adipocytes were affected by the over-expression and by the inhibition of both miR-27a and miR-143. The results indicated that the over-expression of miR-27a could accelerate adipolysis releasing significantly more glycerol and free fatty acids than the negative control (P < 0.001), while the mimic of miR-143 expression, promoted adipogenesis by accumulating more triglycerides (P < 0.001) in the adipocytes. In addition, we demonstrated that there was good correlation (r > 0.98, P < 0.001) between the indicators of adipolysis in cell lysates and in the culture medium.
Keywords: porcine; adipogenesis; adipolysis; miR-27a; miR-143
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MDPI and ACS Style

Wang, T.; Li, M.; Guan, J.; Li, P.; Wang, H.; Guo, Y.; Shuai, S.; Li, X. MicroRNAs miR-27a and miR-143 Regulate Porcine Adipocyte Lipid Metabolism. Int. J. Mol. Sci. 2011, 12, 7950-7959.

AMA Style

Wang T, Li M, Guan J, Li P, Wang H, Guo Y, Shuai S, Li X. MicroRNAs miR-27a and miR-143 Regulate Porcine Adipocyte Lipid Metabolism. International Journal of Molecular Sciences. 2011; 12(11):7950-7959.

Chicago/Turabian Style

Wang, Tao; Li, Mingzhou; Guan, Jiuqiang; Li, Penghao; Wang, Huiyu; Guo, Yanqin; Shuai, Surong; Li, Xuewei. 2011. "MicroRNAs miR-27a and miR-143 Regulate Porcine Adipocyte Lipid Metabolism." Int. J. Mol. Sci. 12, no. 11: 7950-7959.

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