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Int. J. Mol. Sci. 2011, 12(11), 7950-7959; doi:10.3390/ijms12117950

MicroRNAs miR-27a and miR-143 Regulate Porcine Adipocyte Lipid Metabolism

Institute of Animal Genetics & Breeding, College of Animal Science & Technology, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
These authors contributed equally to this work.
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Authors to whom correspondence should be addressed.
Received: 23 September 2011 / Revised: 7 November 2011 / Accepted: 8 November 2011 / Published: 15 November 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

MicroRNAs (miRNAs) are non-coding small RNAs that play roles in regulating gene expression. Some miRNAs have been classed as epigenetic regulators of metabolism and energy homeostasis. Previous reports indicated that the miRNAs miR-27a and miR-143 were involved in lipid metabolism in human and rodents. To determine the roles of porcine miR-27a and miR-143 in adipocyte lipid metabolism, porcine adipocytes were cultured and allowed to induce differentiation for 10 days. The lipid-filled adipocytes were then transfected with miRNA mimics and inhibitors. We measured how the indicators of adipogenesis and adipolysis in porcine adipocytes were affected by the over-expression and by the inhibition of both miR-27a and miR-143. The results indicated that the over-expression of miR-27a could accelerate adipolysis releasing significantly more glycerol and free fatty acids than the negative control (P < 0.001), while the mimic of miR-143 expression, promoted adipogenesis by accumulating more triglycerides (P < 0.001) in the adipocytes. In addition, we demonstrated that there was good correlation (r > 0.98, P < 0.001) between the indicators of adipolysis in cell lysates and in the culture medium. View Full-Text
Keywords: porcine; adipogenesis; adipolysis; miR-27a; miR-143 porcine; adipogenesis; adipolysis; miR-27a; miR-143
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wang, T.; Li, M.; Guan, J.; Li, P.; Wang, H.; Guo, Y.; Shuai, S.; Li, X. MicroRNAs miR-27a and miR-143 Regulate Porcine Adipocyte Lipid Metabolism. Int. J. Mol. Sci. 2011, 12, 7950-7959.

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