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Int. J. Mol. Sci. 2011, 12(11), 7569-7580; doi:10.3390/ijms12117569
Article

Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis

1,2
,
2
,
1
,
1
 and
1,*
1 Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Southeast University, No.87 Dingjiaqiao Road, Nanjing, Jiangsu 210009, China 2 Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China
* Author to whom correspondence should be addressed.
Received: 30 August 2011 / Revised: 19 October 2011 / Accepted: 31 October 2011 / Published: 7 November 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Sustained high concentration of glucose has been verified toxic to β-cells. Glucose augments Ca2+-stimulated insulin release in pancreatic β-cells, but chronic high concentration of glucose could induce a sustained level of Ca2+ in β-cells, which leads to cell apoptosis. However, the mechanism of high glucose-induced β-cell apoptosis remains unclear. In this study, we use a calcium channel blocker, nifedipine, to investigate whether the inhibition of intracellular Ca2+ concentration could protect β-cells from chronic high glucose-induced apoptosis. It was found that in a concentration of 33.3 mM, chronic stimulation of glucose could induce INS-1 β-cells apoptosis at least through the endoplasmic reticulum stress pathway and 10 μM nifedipine inhibited Ca2+ release to protect β-cells from high glucose-induced endoplasmic reticulum stress and apoptosis. These results indicated that inhibition of Ca2+ over-accumulation might provide benefit to attenuate islet β-cell decompensation in a high glucose environment.
Keywords: nifedipine; Ca2+ homeostasis; β-cell; endoplasmic reticulum stress; apoptosis; high glucose nifedipine; Ca2+ homeostasis; β-cell; endoplasmic reticulum stress; apoptosis; high glucose
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wang, Y.; Gao, L.; Li, Y.; Chen, H.; Sun, Z. Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis. Int. J. Mol. Sci. 2011, 12, 7569-7580.

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