Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. MTT Assay
2.4. Hoechst 33342 Staining
2.5. Tunel Staining
2.6. Western Blot Analysis
2.7. Calcium Mobilization Assay
2.8. Insulin Staining and Glucose Stimulated Insulin Secretion (GSIS) Assay
2.9. Statistical Analysis
3. Results
3.1. Nifedipine Protects INS-1 Cells from High Glucose-Induced Apoptosis
3.2. Nifedipine Prevents High Glucose-Induced β-Cell Death Through ER Stress Pathway
3.3. Nifedipine Improves the High Glucose-Impaired Calcium Homeostasis and GSIS in INS-1 Cells
4. Discussion
Acknowledgements
References
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Wang, Y.; Gao, L.; Li, Y.; Chen, H.; Sun, Z. Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis. Int. J. Mol. Sci. 2011, 12, 7569-7580. https://doi.org/10.3390/ijms12117569
Wang Y, Gao L, Li Y, Chen H, Sun Z. Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis. International Journal of Molecular Sciences. 2011; 12(11):7569-7580. https://doi.org/10.3390/ijms12117569
Chicago/Turabian StyleWang, Yao, Lu Gao, Yuan Li, Hong Chen, and Zilin Sun. 2011. "Nifedipine Protects INS-1 β-Cell from High Glucose-Induced ER Stress and Apoptosis" International Journal of Molecular Sciences 12, no. 11: 7569-7580. https://doi.org/10.3390/ijms12117569