Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Aaptamine Inhibited the Proliferation of K562 Cells
2.2. Aaptamine Arrested the Cell Cycle of K562 Cells at G2/M Phase
2.3. Aaptamine Induced p21 Expression in K562 Cells
2.4. Aaptamine Activated p21 Promoter in K562 Cells
3. Experimental Section
3.1. Materials
3.2. Isolation and Identification of Aaptamine
3.3. Cell Culture
3.4. WST-8 Assay to Determine K562 Cell Growth
3.5. Flow Cytometric Analysis of Cell Cycle
3.6. Western Blot Analysis
3.7. Transfection of K562 Cells and Luciferase Assay
4. Conclusions
Acknowledgments
References
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Jin, M.; Zhao, W.; Zhang, Y.; Kobayashi, M.; Duan, H.; Kong, D. Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells. Int. J. Mol. Sci. 2011, 12, 7352-7359. https://doi.org/10.3390/ijms12117352
Jin M, Zhao W, Zhang Y, Kobayashi M, Duan H, Kong D. Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells. International Journal of Molecular Sciences. 2011; 12(11):7352-7359. https://doi.org/10.3390/ijms12117352
Chicago/Turabian StyleJin, Meihua, Wennan Zhao, Yanwen Zhang, Motomasa Kobayashi, Hongquan Duan, and Dexin Kong. 2011. "Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells" International Journal of Molecular Sciences 12, no. 11: 7352-7359. https://doi.org/10.3390/ijms12117352